Basic Fiber Photometry System
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In conventional fiber photometry systems for behaving animals, the fiber-optic rotary joint transfers light signals and allow fiber tether rotation. However, by doing so, the optical joint introduces some transmission losses and rotation-related optical signal variation, which might not be acceptable for all applications. Those drawbacks are eliminated if the so-called Rotary Fluorescence Mini Cube (RFMC) with integrated LEDs and detectors is mounted on a rotating side of a 24-channel Assisted Electrical Rotary Joint. This approach enables both one- and two-site photometry recordings depending on the number of attached cubes.
Currently, we offer LED light sources for GCaMP, GCaMP with Red Fluo, and FRET(CFP/YFP). Customization with other light sources, such as laser diodes and different fluorophore combinations, might be possible if requested.
- The GCaMP Fiber Photometry System measures the 405 nm excited GCaMP fluorescence (isosbestic point), and the 470 nm excited calcium-dependent GCaMP fluorescence, on a single photodetector. The fluorescence emission can be demodulated by lock-in amplifier detection, or by interleaved excitations.
The GCaMP Fiber Photometry System includes :
- Fiber Photometry Console, the core of the system
- 2-channels LED Driver to control LED power
- Fluorescence Mini Cube - GCaMP with built-in LEDs and DETECTOR
- Doric Neuroscience Studio software
Add optional items :
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with parts pre-assembled
- Optical Power meter to monitor and calibrate excitation power
Complete your system with :
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Low autofluorescence optical patch cords to bring light to the animal
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule to ferrule interconnect
NOTE : Other light sources, such as laser diodes, and different fluorophore combinations are possible. Please do not hesitate to request your preferences. - The GCaMP with Red Opsin Fiber Photometry System measures the 405 nm excited GCaMP fluorescence (isosbestic point), and the 465 nm excited calcium-dependent GCaMP fluorescence, on a single photodetector. The fluorescence emission can be demodulated by lock-in amplifier detection, or by interleaved excitations. It also has an optical fiber input port (FC) to connect the light source for red opsin activation or inhibition.
The GCaMP with red opsin Fiber Photometry System includes :
- Fiber Photometry Console, the core of the system
- 2-channels LED Driver to control LED power
- Fluorescence Mini Cube - GCaMP with red opsin with built-in LEDs and DETECTOR
- Doric Neuroscience Studio software
Add optional items :
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with parts pre-assembled
- Optical Power meter to monitor and calibrate excitation power
Complete your system with :
-
Low autofluorescence optical patch cords to bring light to the animal
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule to ferrule interconnect
- Red Laser light source or Ce:YAG source
NOTE : Other light sources, such as laser diodes, and different fluorophore combinations are possible. Please do not hesitate to request your preferences. - The GCaMP and Red Fluorophore Fiber Photometry System measures the 405 nm excited GCaMP fluorescence (isosbestic point), and the 465 nm excited calcium-dependent GCaMP fluorescence, on a single photodetector. The fluorescence emission can be demodulated by lock-in amplifier detection, or by interleaved excitations. It also has a 560 nm excitation LED for red fluorophore excitation with a second detector for red fluorescence recording.
The GCaMP with red fluorophore Fiber Photometry System includes :
- Fiber Photometry Console, the core of the system
- 4-channels LED Driver to control LED power
- Fluorescence Mini Cube - GCaMP with red fluorophore with built-in LEDs and DETECTOR
- Doric Neuroscience Studio software
Add optional items :
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with parts pre-assembled
- Optical Power meter to monitor and calibrate excitation power
Complete your system with :
-
Low autofluorescence optical patch cords to bring light to the animal
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule to ferrule interconnect
NOTE : Other light sources, such as laser diodes, and different fluorophore combinations are possible. Please do not hesitate to request your preferences. -
Fiber Photometry System
- Getting Started Guide, Version 2.2.1
Behavior Camera: Synchronization & Recording
- Application Note, Version 1.0.0
- choosing a selection results in a full page refresh