Fiber Photometry Solutions
The fiber photometry is a technique of monitoring the neuronal activity of neurons labelled with fluorescent reporter(s) in behaving lab animals that uses chronically implanted optical fiber to deliver excitation light and collects induced fluorescence from the targeted brain region.
- Record neural activity of a specific neuron population
- Compatible with deep brain fiber-optic cannula
- Low impact on animal in freely behaving experiment
- Possible to record multiple sites
- High temporal resolution
- High sensitivity, low optical power required
- Spatial resolution limited to the optical fibre core diameter
- Limited sampling depth, due to tissue scattering (1-photon)
- Invasive chronic implant
Doric Lenses offers complete solution for fiber photometry going from data acquisition and analysis software, light sources, detectors, optical filters cubes, patch cords and rotary-joints to fiber-optic cannulas and related accessories.
While each solution has its own advantages, they are all based on a data acquisition console controlled by Doric Neuroscience Studio that takes care of data recording, real-time lock-in amplifier or interleave demodulation with a user intuitive interface requiring no programming skills. The console enables synchronization of the fiber photometry recordings with behavior recordings, optogenetics light stimulation control and/or electrophysiology recordings.
In addition to data acquisition console, Basic setup for fiber photometry consists of LED light source(s) that can provide microWatts (µW) of fluorescence excitation light and a photo-detector(s) sensitive to picoWatts (pW) of fluorescence emission, all packaged within compact Fluorescence Mini Cube (FMC) with appropriate spectral filtering of excitation and emission light and connectorized to a low auto-fluorescence fiber-optic patch cord and fiber-optic rotary joint followed with a chronically implanted fiber-optic cannula.
Different FMC configurations are available to work with 1, 2 or 3 fluorophores and for optogenetics stimulation. The original connectorized FMC cubes are of modular design where an optical fiber is connected to each port to light sources and photodetectors. Newly developed Generation 2 of FMC cubes gets away with optical fibers and some electrical cables wherever possible and integrates LED light sources, photodetectors and their amplifiers into compact unit with improved sensitivity.
For those experiments where the transmission artifacts from optical rotary-joint rotation are not acceptable, we recommend the Rotary-Fluorescence Mini Cube fiber photometry system. It use same equipment as basic system, except the fluorescence minicube that integrate LED and detectors and is redesigned to be attached on the rotor of an assisted electrical rotary-joint; removing the transmission variation in rotation of an optical rotary-joint.
When simultaneous fiber photometry recordings are required for several animals in separate locations, or several sites on the same animal, the simplest and most cost effective solution is the Bundle imaging Fluorescence Mini-Cube fiber-photometry system that can record fluorescence signal from multiple optical fibers in parallel. Optical fibers are bundled together at the end facing CMOS detector while the other end is branching out to individual fibers. When used for freely behaving recordings on several animals in separate location, each fiber end has to be equipped with a fiber-optic rotary joint. If recordings are for several sites on the same animals then the animal has to be restrained from turning around.
A solution to overcome this limitation is to merge the Rotary FMC and Bundle imaging FMC approaches to allow fiber photometry recording of multiple sites in an animal for long term freely-moving experiment. This solution has been developed and should be released later this year.
All our fiber photometry solutions can be combined with other techniques as optogenetics, electrophysiology and miniaturized fluorescence microscopy.
|Comparison Table for Fiber Photometry systems|
|System Type||Basic FMC||Rotary FMC||Bundle imaging FMC||Bundle imaging Rotary FMC|
|Number of sites
|up to 4||up to 4||up to 100||up to 100|
|Number of sites
(per animal, freely-moving)
|2||2||2||up to 100|
|Fluorescent markers||up to 3||up to 2||up to 2||up to 2|
|Different configurations for each site||yes||yes||no||no|
|Combine with Optogenetics
|Reference Baseline Correction||lock-in /
1. Tecuapetla F et al. Balanced activity in basal ganglia projection pathways is critical for contraversive movements.
Nat. Commun. 5, 4315 (2014).
2. Lerner TN et al. Intact-Brain Analyses Reveal Distinct Information Carried by SNc Dopamine Subcircuits.
Cell 30, 635-47 (2015).
3. Kim CK, et al. Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain.
Nat Methods 13, 325-8 (2016)
|4. Deisseroth lab Clarity Ressource Center|
|5. Fiber Photometry forum|