Fiber Photometry with Fluorescence Mini Cubes (1-2 sites)
The role of Fluorescence Mini Cubes (FMC), a pre-assembled system of filters, optical collimators, and FC receptacles (ports), is to combine excitation light and separate fluorescence light. The FMC classification is based on the number of ports from 3 to 7. Initially, all ports used optical fibers to connect to light sources, detectors, and a sample under test. However, eliminating fiber connections to detectors and LED light sources significantly improves signal detection while making the setup simple, more compact, and less fragile. For example, attaching the Fluorescence Detector directly to FMC results in a 30% increase in signal transmission. A 2-nd generation FMC with built-in LED(s), detector(s), and their amplifiers has fewer electrical connections and only one fiber link leading to the sample. We still offer cubes with FC receptacles on all ports and those with built-in detectors for legacy reasons.
The one-site fiber photometry experiments may require an FMC with a different number of excitation and detection channels, optional optogenetic activation/silencing channel, etc., directly affecting the FMC ports count. The fiber-optic tether leading to the behaving animal needs a Pigtailed 1x1 Fiber-optic Rotary Joint, which has less signal variation. The two-site fiber photometry requires two FMC cubes and, in the case of behaving animal, a Pigtailed 2x2 Fiber-optic Rotary Joint.