VoluScan™ Gen.2

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  • VoluScan™ is an epifluorescence mesoscope that enables 1- or 2-color functional imaging of large sample volumes at a cellular resolution.​ VoluScan™ is designed to image fluorescent sensors (like GCaMP, jRGECO, dLight, etc.) in large 3D biological samples such as organoids, assembloids, brain slices, and cell cultures, but can also be used to study cell migration, division, or other processes. Furthermore, the system is compatible with Tokai Hit incubation chamber and can synchronize imaging with other systems using external digital triggers. Thus, VoluScan™ pairs readily with Light Sources (for optogenetics or uncaging), electrophysiology recordings, liquid infusion, etc.

    Gen.2 of VoluScan is fitted with a motorized 3D stage to sequentially image multiple regions of large sample plates (well culture plates, Petri dishes, …) for high-throughput imaging. Simply leaving VoluScan overnight after defining regions of interest can greatly accelerates data collection.

    The VoluScan™ has an extensive field of view (up to 5 mm) with subcellular resolution (2.7 µm), and covers a large depth (up to 1.5 mm). Specifically, the VoluScan™'s multi-plane imaging system enables scanning such volumes at high sampling rates:

    - 4-planes (1-color) at 16 FPS

    - 8-planes (1-color) at 10 FPS

    Each VoluScan system includes a VoluScan's multi-plane disc that determines the number of imaging planes (4 or 8), the z-stack steps, and the total imaging depth. By default, the z-stack increments  are available in 50 µm, 100 µm or 150 µm steps (others on custom request). These discs are interchangeable to fit the needs of different experiments and can be purchased separately.

    Check out the first bioRiv publication of assembloid data collected with VoluScan™™️ Gen.1. Miura et al. (2024), used the VoluScan's large FOV and fast z-scanning capabilities to image the live, calcium dynamics of over 400 cells per assembloid and demonstrated a functioning model of the cortico-striatal-thamalic-cortical circuit. Learn more


  • General properties:
    Field of view
    3.2 mm x 5.0 mm
    Objective NA
    0.16
    Magnification
    2.2x
    Working distance 5 mm
    Configuration inverted*
    Mechanicals stage travel X: 25 mm
    Y: 25 mm
    Z: 12.5 mm
    Dimensions 210 x 320 x 400 mm
    Computer interface 1x USB2.0 port + 1x USB3.0 port
    Z stack properties
    Number of planes 4, 8
    Step
    50, 100 or 150 µm**
    Maximum depth range
    1.5 mm
    Sensor
    Type
    CMOS Image Sensor***
    Quantum efficiency
    82% at 520 nm
    Resolution 1920 x 1200 pixels
    Pixel Size 5.86 µm x 5.86 µm
    1-color
    Spectral bandwidth
    Excitation: 460-490 nm
    Emission: 500-550 nm
    Maximum framerate (full FOV)
    1 plane: 80 FPS
    4 planes: 16 FPS
    8 planes: 10 FPS
    2-color
    Spectral bandwidth Excitation #1: 400-410 nm or 410-420 nm
    Excitation #2: 555-570 nm

    Emission #1: 500-540 nm
    Emission #2: 580-680 nm
    Maximum framerate (full FOV) 1 plane: 40 FPS
    4 planes: 8 FPS
    8 planes: 5 FPS
    DI/O
    Number of ports
    4
    Input voltage 0 to 5.0 V (Maximum)
    TTL level HI > 3.3 V
    LOW < 1.5 V
    Output voltage TTL 5.0 V (High impedance)
    Output Current max 20 mA per channel
    Maximum sampling rate
    10 kSps
    Maximum output frequency
    5 kHz
    *   compatible with Tokai Hit incubation chamber
    ** other value on custom request
    ***Andor sCMOS on custom request
  • Drawing

    VoluScan™ Gen.2