Fiber Photometry

In neuroscience, the fiber photometry denotes a method where the optical fiber(s) chronically implanted near the targeted brain region of interest expressing calcium indicator(s) delivers excitation light and collects overall fluorescence induced by calcium-activity during the light excitation. While the fluorescence microendoscopy records activity of individual neurons within the field of view, the fiber photometry sums up the overall fluorescence of neurons expressing a genetically encoded calcium indicator. The typical setup for freely behaving animals consists of the excitation light source, the beamsplitter/combiner that separates excitation and fluorescence light, the fiber-optic rotary joint, the optical cannula and connecting fiber-optic patch cords.