Electrophysiology Solutions

Electrophysiology is a wide field of research that study electrical properties of biological tissues.  In neuroscience, it is typically referred to as a technique that measures neurons electrical activities by introducing electrodes into biological tissues

  • Advantages:
    • Direct report of cell electrical activity
    • Large range of recording (single unit, Local field potential, EEG, ...)
    • High signal-noise ratio
    • High temporal resolution
  • Disadvantages:
    • Low neuron type specificity
    • Invasive

Solutions

Back in 2010, Doric Lenses started to offer electrodes assembled with an optical fiber for chronic extracellular electrophysiology recording combined with optogenetics manipulations. Since then, our offering of electrophysiology components has increased to respond to a growing demand for integrated solutions that can combine electrophysiology and optical technique. We are now proud to offer a full range of solutions to add extracellular electrophysiology recording to optical techniques like optogenetics, fiber photometry, and miniature fluorescence microscopy.

Our first group of electrophysiology solutions is for acute recording into non-moving in-vivo animals. Doric Lenses offers an elegant solution with a replaceable probe tip that combines a multimode optical fiber for multi-unit illumination and recording with a wire electrode precisely positioned side by side.

For acute recording where optical single-cell resolution is desired, we have another type of probe based on an optical fiber having an optical fiber core and a second hollow core to be filled with electrolyte to use as an electrode. These probes have tapered tip with lateral dimension down to 10 µm to ensure that optical measurement or manipulation is done at a single-cell scale.

For long-term experiments with freely moving animals, we offer chronically implantable optical fibers pre-assembled with one or multiple wires electrodes.  Our tethered system allows an optical connection to our Doric optogenetics or fiber photometry systems via optical fiber patch cords, while electrodes are connected to an headstage that does amplification, analog-to-digital conversion, and transfer recording signal to the computer by small wire electrical cable.

 

We also offer a wireless freely moving option. In this system, the optical fiber is directly connected to a LED within a chronic implant, which allows triggered optical stimulation with selected intensity and time patterns. The extracellular electrical activity is recorded using electrodes and a battery-driven headstage. The wireless link between the animal and the control station is assured by radiofrequency antennas (FiWi system).


Comparison Table for Doric Lenses Electrophysiology systems
System Type Acute multi-units Acute single-cell Tethered Wireless
Number of optical fiber
1 1 1 or 2 1
Number of electrodes 1 to 8 1 1 to 16 1 to 4
Compatible with Freely-Moving no no yes yes
Combine with Optogenetics yes yes yes yes
Combine with Fiber-photometry yes yes yes no
Combine with Miniature fluorescence microscopy
no no yes no
Availability now now soon now



External References

1. Scanziani, M., Häusser, M. Electrophysiology in the age of light.
    Nature 461, 930–939 (2009).
2. LeChasseur Y, et al., A microprobe for parallel optical and electrical recordings from single neurons in vivo. 
    Nature Methods 8, 319–325 (2011)
3. Dufour S, et al., A Multimodal Micro-Optrode Combining Field and Single Unit Recording, Multispectral Detection and Photolabeling Capabilities. 
    PLoS ONE 8, e57703 (2013)
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4. Ameli R, et al., A wireless and batteryless neural headstage with optical stimulation and electrophysiological recording.
    Conf Proc IEEE Eng Med Biol Soc. 5662-5 (2013).
5. Gagnon-Turcotte G. et al., A wireless optogenetic headstage with multichannel electrophysiological recording capability.
    Sensors 15, 22776-22797 (2015).