VoluScan™

VoluScan™

VoluScan™ is a fluorescent mesoscope that enables 1- or 2-color functional imaging of large sample volumes at a cellular resolution. VoluScan™ is designed to image fluorescent sensors (like GCaMP, jRGECO, dLight, etc.) in organoids, assembloids, brain slices, and cell cultures, but can also be used to study cell migration, division, or other processes. Furthermore, the system is compatible with Tokai Hit incubation chamber and can synchronize imaging with other systems using external digital triggers. Thus, VoluScan™ pairs readily with Light Sources (for optogenetics or uncaging), electrophysiology recordings, liquid infusion, etc.

VoluScan™ has an extensive field of view (up to 5 mm) with subcellular resolution (2 µm), and covers a large depth (up to 1.5 mm). Specifically, the VoluScan™'s multi-plane imaging system enables scanning such volumes at high sampling rates.

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VoluScan™ is a fluorescent mesoscope that enables 1- or 2-color functional imaging of large sample volumes at a cellular resolution.​ VoluScan™ is designed to image fluorescent sensors (like GCaMP, jRGECO, dLight, etc.) in organoids, assembloids, brain slices, and cell cultures, but can also be used to study cell migration, division, or other processes. Furthermore, the system is compatible with Tokai Hit incubation chamber and can synchronize imaging with other systems using external digital triggers. Thus, VoluScan™ pairs readily with Light Sources (for optogenetics or uncaging), electrophysiology recordings, liquid infusion, etc.

 The VoluScan™ has an extensive field of view (up to 5 mm) with subcellular resolution (2 µm), and covers a large depth (up to 1.5 mm). Specifically, the VoluScan™'s multi-plane imaging system enables scanning such volumes at high sampling rates:

  • - 4-planes (1-color) at 16 FPS
  • - 8-planes (1-color) at 10 FPS

 Each VoluScan system includes a VoluScan's multi-plane disc that determines the number of imaging planes (4 or 8), the z-stack steps, and the total imaging depth. By default, the z-stack increments  are available in 50 µm, 100 µm or 150 µm steps (others on custom request). These discs are interchangeable to fit the needs of different experiments and can be purchased separately.

Check out the first bioRiv publication of assembloid data collected with VoluScan™. Miura et al. (2024), used the VoluScan's large FOV and fast z-scanning capabilities to image the live, calcium dynamics of over 400 cells per assembloid and demonstrated a functioning model of the cortico-striatal-thamalic-cortical circuit. Learn more

General properties:
Field of view
3.2 mm x 5.0 mm
Objective NA
0.16
Magnification
2.2x
Working distance5 mm
Configurationinverted*
Mechanicals stage travelX: 25 mm
Y: 25 mm
Z: 12.5 mm
Dimensions210 x 320 x 400 mm
Computer interface1x USB2.0 port + 1x USB3.0 port
Z stack properties
Number ofplanes4, 8
Step
50, 100 or 150 µm**
Maximum depth range
1.5 mm
Sensor
Type
CMOS Image Sensor***
Quantum efficiency
82% at 520 nm
Resolution1920 x 1200 pixels
Pixel Size5.86 µm x 5.86 µm
1-color
Spectral bandwidth
Excitation: 460-490 nm
Emission: 500-550 nm
Maximum framerate (full FOV)
1 plane: 80 FPS
4 planes: 16 FPS
8 planes: 10 FPS
2-color
Spectral bandwidthExcitation #1: 400-410 nm or 410-420 nm
Excitation #2: 555-570 nm
Emission #1: 500-540 nm
Emission #2: 580-680 nm
Maximum framerate (full FOV)1 plane: 40 FPS
4 planes: 8 FPS
8 planes: 5 FPS
DI/O
Number of ports
4
Input voltage0 to 5.0 V (Maximum)
TTL levelHI > 3.3 V
LOW < 1.5 V
Output voltageTTL 5.0 V (High impedance)
Output Currentmax 20 mA per channel
Maximum sampling rate
10 kSps
Maximum output frequency
5 kHz
*compatible with Tokai Hit incubation chamber
** other value on custom request
***Andor sCMOS on custom request
Drawing

VoluScan™



The VoluScan™ system for GCaMP imaging includes:

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