Data Analysis Solution for Fiber Photometry
Accelerate your fiber photometry and behavior analyses!
All-in-one solutions:
- Convert to Doric format
- Import behavior data
- Create Analysis Pipelines
- Pool data by conditions
- Export figures
Accelerate analysis
User-friendly interface
Expert Support
No coding!
danse™ is a powerful data analysis software tailored for neuroscientists using fiber photometry and behavioral data.
With intuitive workflows, create pipelines that automate multiple operations (Remove artifacts, z-ΔF/F0, Find Spikes, PeriEvent, etc.), without any coding required. The software also imports and aligns behavior data from non-Doric sources (videos, csv, DIO, etc.).
Beyond single recording analyses, danse™ easily pools multi-animal data according to your experimental design and re-run analyses effortlessly with new parameters—saving time and accelerating publications.
Processing and analysis operations
danse™ offers a growing set of tools and operations, including fiber photometry, behavioral data, data conversion, and advanced analysis operations. Browse the categories below for an overview of the most popular operations and their tutorial videos. New operations are added reguarly based on user requests.
Create an Experiment Project
danse™ provide a simple framework to organise all your fiber photometry & behavior data. Save all the metadata related to each recording (Animal ID, sex, genotype, batch, etc.) and the experimental design (group, treatment, trial, task, etc.).
From the Experiment project, you can:
1. Build Pipeline - run several operations over all your data files
2. Link behavior data - quickly link the right CSV or video data to the right fiber photometry file
3. Group Analysis - pool data together according to your experimental design
Conversion Operations
What fiber photometry data is compatible with danse™ software?
Many different types of fiber photomery data are compatible with danse™, including Tucker Davis Technology (TDT), Neurophotometrics, RWD and any cusotm systems using the .csv/.xlsl formats.
However, non-doric data must be converted into the .doric format.
Check out the following tutorial videos to learn how to convert your data:
Fiber Photometry Operations
Over a dozen fiber photometry operations are available and can be selected for batch processing, including remove artifcats, remove baseline, filter, interpolation, resampling, smooth signals, several different ways to calculate ∆F/F, find spikes, standardization, and many more...
Here are a few highlights and their tutorial videos:
Behavior Operations
Danse™ provides a large number of behavior operations for both Doric and non-doric behavior videos and digital/analog signals. For those recording behavior with Doric Neuroscience Studio, can directly process behavior in danse™.
Import Behavior Data
If your behavior data was not recorded in Doric Neuroscience Studio, use the following operations to import behavior data into your existing .doric file/experiment project. These operations are compatible for common behavior recording systems: Noldus, ANY-Maze, MedAssociate, DeepLabCut, etc. and in different formats (video or csv file).
Several sychronization methods are available to insure the behavior and neural data are well aligned to each other.
TUTORIAL VIDEOS:
Calculate Animal Coordinates
Whether importing videos or using Doric Behavior Camera or CamLoop, Danse™ can directly process animal behavior within the software. Many of the behavior operations (calculate speed, prescence in zone, or distance from point) required animal coordinates as the imput.
Danse™ provides several operations to calculate animal coordinates, including Animal Tracking and Pose Estimation operations.
Animal Tracking
Pose Estimation
Next, the animal coordinates can be used to compute the following measures.
Calculate speed
Presence in zone
Distance from point
Each measure (speed, etc.) can be fed into the Create Behavior Events section.
Create Behavior Events
This operation outputs timestamps of behavior or stimuli that occured during the fiber photometry recording. These timestamps can be only the onset, only the offset or onset-offset pairs.
These timestamps are called Events in danse and are important for operation combining neural and behavior data together.
Behavior events can be created from imported .csv data, manually-entered timepoints, from recorded digital (TTL) or analog signals, or from measures created in danse™(speed, motion score presence in zone, distance from point, etc.).
Behavior events are required inputs for PeriEvent and Signal Statistics plots.
Here are examples of both digital and continuous measures converted into events.
Create Time Periods
Use Create Time Periods feature to examine how neural activity changes over time and/or to grad a subset of behavior events ocurring at certain time points.
Manual Time Periods:
Define windows of varying lengths where the animal encoutered or experianceed different conctexts (Baseline vs manipulation, new cage, drug injection, etc.).
Auto-Time Periods:
Divide the entire recording into even time windows (seconds, minutes, hours).
Analysis Operations
Analysis operations are the core strength of the danse™ software, providing simple way to combine behavior and neural data together. Specifically, these operations offer different ways of quantifying how neural activity changes around key behavior time points.
Perievent Operation
Perievent Histograms (also called peri-stimulus time histograms or PSTHs) visualizes changes in neural activity in response to the onset (or offset) of a stimulus or behavior of interst (i.e event). This plot is calculated by aligning and averaging the ΔF/F0 centered arount that. This results in a histogram that shows the average neural response over time.
Statistics associated to the PeriEvents trace are also calculated, including Aera-under-the-curve (AUC), maximum, minimum, etc.
Plot perievent as a heatmap. For single animal perievents, each row corresponds to an event (trial/bout), while for group analysis perievents, each row corresponds to the average trace of one animal.
Spatial Signal Operation
The spatial signal operation combines neural activity and animal tracking data into a heatmap.
The x and y axes of the heatmap corespond to the cage dimensions and the color is associated with the average ΔF/F0 signal when the animal was in this location.
Spatial Signal operation is ideal for analysis such as condition-place preference, Plus-maze, novel object, animal interactions and hippocampal place cells, among many others.
Signal Statistics Operation
The Signal Statistic operation examines changes in neural activity over the duration of the entire duration of an event/time preiod (i.e not just the onset/offset of the event). This operation is ideal for behaviors of varying lengths (freezing, entering zone, animal interactions, etc.).
Calculate the following statistics:
- z-ΔF/F0 : AUC, mean, median, max, min
- Spikes : amplitude, firing rate, # spikes
Export Figures & Data
danse™ allows for easy data export, either as figures (.pdf, .png, .jpeg) or as .csv files.
Automate fiber photometry analyses WITHOUT coding!
Create Experiment Project
Build Pipeline
Group Analysis
Import Behavior
