Fluorescence Mini Cube - GCaMP with Red fluorophore (6 ports)

Fluorescence Mini Cube - GCaMP with Red fluorophore (6 ports)
  • Unit price per 

Ordering Code:
  • This cube can be used to detect the fluorescence from two calcium indicators and the GCaMP isosbestic point. The separation of functional and isosbestic fluorescence signals of GCaMP is possible if the light sources are modulated.

    In order to optimize signal detection and simplify usage, it is desirable to minimize the number of fiber optic connections to a Fluorescence Mini Cube. By taking advantages of the new Doric Fluorescence Detector, and our Built-in LED Optical Heads, it is possible to have an Integrated Fluorescence Mini Cube.

    • The Built-in Fluorescence Detector allows for an increase in signal transmission of 30 % due to the proximity of the detector active surface and signal source, as well as the reduced number of optical connections. A Fluorescence Detector Amplifier is provided for each detector head.

    • The Built-in LED Optical Head removes the need for light source patch cords. The Intensity Adjustment Ring allows additional fine control of light intensity, ideal for low-power fiber photometry experiments.

    On the image E is for excitation, F for fluorescence and S is for the fixed sample port.

    The 540-570 nm Excitation Band Built-in LED Optical Head does not include an Intensity Adjustment Ring.

  • Mini Cube
    Wavelength range 350 to 1100 nm
    Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
    Spectral bandwidth GCaMP :
    - Isosbestic Excitation : 400-410 nm
    - Excitation 460-490 nm

    - Emission 500-550 nm

    Red Fluorophore :
    - Excitation 540-570 nm
    - Emission 580-680 nm
    Optical filter attenuation > OD 5 outside band
    Dimensions 57 mm x 63 mm x 26 mm
    Built-in Detectors
    Detector Sensitivity - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
    Amplification 3 levels : 1x, 10x, 100x
    Transimpedance gain - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    - 2.0 E11 V / A (100x)
    Conversion gain
    @ 550 nm
    - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    - 76.0 V / nW (100x)
    Saturation level
    @ 550 nm
    - 7.2 nW (1x)
    - 0.72 nW (10x)
    - 72 pW (100x)
    NEP < 12 fW/√Hz
    Bandwidth - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
    Electrical interface BNC
    Output Voltage 0.0 to 5.5 V
    Output impedance 50 Ω
    Sensor type Silicon photodiode
    Sensor Area 1 x 1 mm
    Input voltage 12 V DC (power supply included)
    Built-in LEDs
    Max Current 200 mA
    Emitter size 400 µm diameter - NA0.22
    Max Output Power
    (fibre 400 µm)
    LED 460-490 nm
    = 1.5 to 120 µW (adjustable)
    LED 540-570 nm
    = 50 µW (fixed)
  • FMC6 DRAWING
      (FC connector on all ports)
    iFMC6 (Gen 2) DRAWING
      (Built-in DETECTOR Gen.2)
    ilFMC6 (Gen 2) DRAWING
      (Built-in LED and DETECTOR Gen.2)
    iFMC / ilFMC_GEN2 USER MANUAL
      Built-in detector Gen.2
    FMC USER MANUAL
      FC connector on all ports
    iFMC Gen.1 vs. Gen.2 comparison
      Built-in detector FMC compare Gen.2 vs. Gen.1
    iFMC / ilFMC GEN 1 USER MANUAL
        Built-in DETECTOR - Gen 1