7 ports Fluorescence Mini Cube - GCaMP, Red fluorophore and Optogenetics

7 ports Fluorescence Mini Cube - GCaMP, Red fluorophore and Optogenetics
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  • This cube combines the detection of two calcium indicators (GCaMP and red fluorophores) with the activation of red opsins. It integrates dichroic mirrors to combine fluorophores excitations, isolate the fluorescence emissions, and let opsin stimulation light through. At the same time, narrow bandpass filters limit the excitation and detection spectral bands:

    • 400-410 nm or 410-420 nm for GCaMP isosbestic point excitation
    • 460-490 nm for GCaMP excitation
    • 500-540 nm for GCaMP fluorescence detection
    • 555-570 nm for red fluorophore excitation
    • 580-600 nm for red fluorescence detection
    • 628-642 nm for red opsins activation


    Compared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, in the latest generation of Mini Cubes, LEDs and LED drivers are integrated as well to reduce the number of connections and the system’s overall footprint. In his generation, it is also possible to replace the excitation filter and decrease the excitation power by adding attenuating filters (for low power photometry).

  • Mini Cube
    Wavelength range 350 to 1100 nm
    Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
    Spectral bandwidth

    GCaMP :
    - Isosbestic Excitation : 400-410 nm or 410-420 nm
    - Excitation 460-490 nm

    - Emission 500-540 nm

    Red Fluorophore :
    - Excitation 555-570 nm
    - Emission 580-600 nm

    Optogenetic :
    - Excitation 628-642 nm

    Optical filter attenuation > OD 5 outside band
    Dimensions 152 mm x 55 mm x 68 mm
    Built-in Detectors
    Detector Sensitivity - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
    Amplification 3 levels : 1x, 10x, 100x
    Transimpedance gain - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    - 2.0 E11 V / A (100x)
    Conversion gain
    @ 550 nm
    - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    - 76.0 V / nW (100x)
    Saturation level
    @ 550 nm
    - 7.2 nW (1x)
    - 0.72 nW (10x)
    - 72 pW (100x)
    NEP < 12 fW/√Hz
    Bandwidth - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
    Electrical interface BNC
    Output Voltage 0.0 to 5.5 V
    Output impedance 50 Ω
    Sensor type Silicon photodiode
    Sensor Area 1 x 1 mm
    Input voltage 12 V DC (power supply included)
    Built-in LEDs
    Max Current 500 mA
    Emitter size 400 µm diameter - NA0.22
    Max Output Power
    (fibre 400 µm)
    LED 460-490 nm
    = 130  µW (at 200 mA)
    LED 540-570 nm
    = 130  µW (at 200 mA)
  • Fluorescence Mini Cube 7 ports (FC connector on all ports)
      Drawing
    Fluorescence Mini Cube (FC connector on all ports)
      User Manual

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