Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - System

Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - System

Rotary Bundle-imaging fiber photometry (RBFMC-G2) is an elegant alternative for long-term, multiple-site recordings in freely-moving animals. This CMOS-based photometry system is fixed onto the motorized rotary joint, allowing signal detection during the rotation. This design completely abolishes rotation artifacts and is ideal for recording subtle signals from sparse neural populations and/or from axons.

Rotary Bundle-imaging fiber photometry (RBFMC-G2) is an elegant alternative for long-term, multiple-site recordings in freely-moving animals. This CMOS-based photometry system is fixed onto the motorized rotary joint, allowing signal detection during the rotation. This design completely abolishes rotation artifacts and is ideal for recording subtle signals from sparse neural populations and/or from axons.

Both Fan-out and High-density bundle patch cords are interchangeably compatible with the Sample port of the same RBFMC-G2. The Fan-out option is designed for multiple distant brain regions, each fitted with Mono Fiber-optic cannula, while the High-density type is designed for High-density cannulas

RBFMC-G2 configurations:
  1. Two-color photometry (Green & Red + isosbestic)
  2. One-color photometry (Green + isosbestic) with red-shifted optogenetics (a single red LED (wavelength) illuminates all the photometry sites, simultaneously)

Note: it is possible to swap between the two configurations by changing the optical mini cube connected to the rotary joint.

In addition, both RBFMC-G2 configurations are compatible with an additional optogenetic site. This option mounts a laser (one of 6 wavelength options) on the outside of the optical cube for optogenetic stimulation on non-photometry sites.

RBFMC-G2 also has an HDMI port to record electrophysiological signals from the animal with Doric's electrophysiology consoles (EPC, NC500). It is also possible to purchase an adaptor for common electrophysiology systems (Intan, Neuropixel, etc.). Thus, with this state-of-the-art system, users can simultaneously record multi-site photometry, optogenetics, and electrophysiology signals from the same, freely-moving animal.

The GCaMP with Red Fluorophore Rotary Bundle-imaging Fiber Photometry System Gen.2 contains all items necessary to perform fiber photometry measurements on many sites with GFP-like fluorophores. It also measures the isosbestic excitation of GCaMP fluorescence and collects the red fluorescence on the same sensor. The fluorescence resulting from different excitations are demodulated by sequential acquisition.


The system includes:

Optional items:

Accessories:

The GCaMP with Red Opto Rotary Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on many sites with GFP-like fluorophores and optogenetics stimulation of red shifted opsins in the same sites.
 

The system includes:


Optional items


Accessories:

The External optogenetics option fixes a small Laser diode module (CLDM) onto the rotor portion of the RBFMC, providing an additional optogenetic-only stimulation. Both the 2-color (GCaMP + RFP) and the 1-color (GCaMP + Red Opto) configurations are compatible with the external optogenetics

The integrated laser driver inside the RBFMC-G2 (E4 port) controls the timing and intensity independent to the internal fiber photometry/optogenetics.

All six wavelengths of the Laser diode module (CLDM) can be used with the RBFMC-G2. However, we strongly recommend using the 638nm laser, as it works well with ChrimsonR opsin.

External Optogenetic items:

Accessories

This is the only rotary joint solution for simultaneous, multi-site (3+) fiber photometry and electrical recordings. The RBFMC-G2 comes, by default, with two electrical connectors that support electrophysiology(up to 12 pins) or EGG/EMG. The ports can also be used to add custom electrical components (IMU, etc.).

The RBFMC-G2 is designed to work with the NC500 for simultaneous bundle-imaging and electrophysiology recordings. This solution provides a single interface to record both signal types in perfect synchronization. The NC500 system uses either 16-channel or 32-channel Intan RDH headstages and is compatible with Doric multi-electrode optoelectric cannulas (OEC-Multi), with up to 18 pins.

Note that the connector types on the RBFMC are different, depending on their location

  • Rotor Side: PZN12
  • Stator Side: HDMI

*Adaptors available for compatibility with non-Doric electrophysiology system

Doric Electrophysiology system items

Intan Electrophysiology

Neuropixel Electrophysiology adaptors

 GCaMP + RFPGCaMP + Red Opto
Wavelength range350 to 1100 nm
Field of viewØ1.30 mm
Objective NA0.50
Maximum number of sites 
* could be limited by patchcord manufacturing		- 7x core 400 µm NA0.57 
- 20x core 200 µm NA0.57
- 90x core 100 µm NA0.37
Excitation Uniformity10% over FOV
Optical fiber compatibility

- Core diameter 100, 200, or 400 µm
- NA 0.37 to 0.57

Spectral bandwidth- Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
- Excitation #2 : 460-490 nm
- Excitation #3 : 555-570 nm
- Emission #1 : 500-550 nm
- Emission #2 : 580-680n
 		- Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
- Excitation #2 : 460-490 nm
- Excitation #3 : 580-650 nm
- Emission #1 : 500-540 nm
Optical filter attenuation> OD 5 outside band
Optical fiber connectorSMA
Dimensions102 mm x 102 mm x 346mm
SENSOR
TypeCMOS Image sensor
 
Resolution
 		540x 540 px
 
Pixel Size
 		6.9µm x6.9 µm
 
Frame rate
 		up to 125 Hz (1 & 2 excitations)
up to 60 Hz (3 excitations)
 
Computer interfaceUSB 3.0
 
Build-in LEDs
Max Current500 mA (LED 400-410 nm or 410-415 nm or 421-433 nm or 433-445 nm, LED 460-490 nm)
 
1000 mA (LED 555-570 nm)
 		1000 mA (LED Opto 600-640 nm)
Max Output Power
(400 µm fiber, NA 0.57)		LED 400-410 nm or 410-415 nm = 60µW (at 200 mA, CW)
 
LED 460-490 nm = 230 µW (at 200 mA, CW)
 
LED 555-570 nm = 150 µW (at 200 mA, CW)
 		LED Opto 600-640 nm = 7 mW (at 1000 mA, CW)
Additional Optogenetic site
ConnectorM8
 
Max Current1000 mA
Electrophysiology
ConnectorPZN12 (rotor)
HDMI (stator)
Drawing

Rotary Bundle-imaging Fluorescence Mini Cubes - rotary joint

Rotary Bundle-imaging Fluorescence Mini Cubes - Cube

Application Notes

GETTING STARTED: Bundle-imaging Systems

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