Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - System

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DESCRIPTION
GCaMP + RFP
GCaMP + Red Opto
SPECIFICATIONS
SUPPORT

Rotary Bundle-imaging fiber photometry (RBFMC-G2) is an elegant alternative for long-term, multiple-site recordings in freely-moving animals. This CMOS-based photometry system is fixed onto the motorized rotary joint, allowing signal detection during the rotation. This design completely abolishes rotation artifacts and is ideal for recording subtle signals from sparse neural populations and/or from axons.
Both Fan-out and High-density bundle patch cords are interchangeably compatible with the Sample port of the same RBFMC-G2. The Fan-out option is designed for multiple distant brain regions, each fitted with Mono Fiber-optic cannula, while the High-density type is designed for High-density cannulas.

RBFMC-G2 configurations:
1. Two-color photometry (Green & Red + isosbestic)
2. One-color photometry (Green + isosbestic) with red-shifted optogenetics (a single red LED (wavelength) illuminates all the photometry sites, simultaneously)
Note: it is possible to swap between the two configurations by changing the optical mini cube connected to the rotary joint.

In addition, both RBFMC-G2 configurations are compatible with an additional optogenetic site. This option mounts a laser (one of 6 wavelength options) on the outside of the optical cube for optogenetic stimulation on non-photometry sites.

RBFMC-G2 also has an HDMI port to record electrophysiological signals from the animal with Doric's electrophysiology consoles (EPC, NC500). It is also possible to purchase an adaptor for common electrophysiology systems (Intan, Neuropixel, etc.). Thus, with this state-of-the-art system, users can simultaneously record multi-site photometry, optogenetics, and electrophysiology signals from the same, freely-moving animal.
The GCaMP with Red Fluorophore Rotary Bundle-imaging Fiber Photometry System Gen.2 contains all items necessary to perform fiber photometry measurements on many sites with GFP-like fluorophores. It also measures the isosbestic excitation of GCaMP fluorescence and collects the red fluorescence on the same sensor. The fluorescence resulting from different excitations are demodulated by sequential acquisition.

The system includes:
Optional items:
Accessories:
The GCaMP with Red Opto Rotary Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on many sites with GFP-like fluorophores and optogenetics stimulation of red shifted opsins in the same sites.

The system includes:
Optional items
Accessories:

	GCaMP + RFP	GCaMP + Red Opto
Wavelength range	350 to 1100 nm
Field of view	Ø1.30 mm
Objective NA	0.50
Maximum number of sites * could be limited by patchcord manufacturing	- 7x core 400 µm NA0.57 - 20x core 200 µm NA0.57 - 90x core 100 µm NA0.37
Excitation Uniformity	10% over FOV
Optical fiber compatibility	- Core diameter 100, 200, or 400 µm - NA 0.37 to 0.57
Spectral bandwidth	- Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 555-570 nm - Emission #1 : 500-550 nm - Emission #2 : 580-680n	- Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 580-650 nm - Emission #1 : 500-540 nm
Optical filter attenuation	> OD 5 outside band
Optical fiber connector	SMA
Dimensions	102 mm x 102 mm x 346mm
SENSOR
Type	CMOS Image sensor
Resolution	540 x 540 px
Pixel Size	6.9 µm x 6.9 µm
Frame rate	up to 60 Hz (1 excitation) up to 30 Hz (2 excitations)
Computer interface	USB 3.0
Build-in LEDs
Max Current	500 mA (LED 400-410 nm or 410-415 nm or 421-433 nm or 433-445 nm, LED 460-490 nm)
	1000 mA (LED 555-570 nm)	1000 mA (LED Opto 600-640 nm)
Max Output Power (400 µm fiber, NA 0.57)	LED 400-410 nm or 410-415 nm = 60 µW (at 200 mA, CW)
	LED 460-490 nm = 230 µW (at 200 mA, CW)
	LED 555-570 nm = 150 µW (at 200 mA, CW)	LED Opto 600-640 nm = 7 mW (at 1000 mA, CW)
Additional Optogenetic site
Connector	M8
Max Current	1000 mA
Electrophysiology
Connector	PZN12