efocus Fluorescence Microscopy System Gen 3

efocus Fluorescence Microscopy System Gen 3

The 3rd generation efocus Fluorescence Microscope enables calcium imaging with cellular resolution over a larger brain area of freely-moving behaving animals. Its field of view has increased to 650 x 650 µm, and the electronic depth adjustment is 300 µm. A more sensitive sensor of the 3-rd generation microscope requires less fluorescence excitation light, reducing photobleaching and phototoxicity. The microscope body attaches to the imaging cannula with a simple barrel rotation. No tools are required.

The 3rd generation efocus Fluorescence Microscope enables calcium imaging with cellular resolution over a larger brain area of freely-moving behaving animals. Its field of view has increased to 650 x 650 µm, and the electronic depth adjustment is 300 µm. A more sensitive sensor of the 3-rd generation microscope requires less fluorescence excitation light, reducing photobleaching and phototoxicity. The microscope body attaches to the imaging cannula with a simple barrel rotation. No tools are required.

Drawing

efocus Fluorescence Microscope System Gen 3 with LISER™
User Manual FR EN

efocus Fluorescence Microscope System Gen 3
Application Note FR EN

Laser Safety Information

The GCaMP efocus fluorescence microscopy system Gen 3 is optimized for calcium imaging of GFP-like fluorescence proteins.



The system includes:
Optional items:
Accessories:
Field of View 650 µm x 650 µm
Working distance adjustment range 0 - 300 µm
Frame rate up to 45 fps
Pc connection USB 3.0
Computeur requirements Desktop Computer recommended, Intel Core-i7, 8 GB RAM, Graphic card with 2 GB memory and OpenGL v4.6 compatible
Fluorescence recording
Excitation 458 / 35 nm
Emission 525 / 40 nm

The GCaMP + Red Opsins efocus microscopy system Gen 3 is optimized for calcium imaging of GFP-like fluorescence proteins and activation of Red-shifted Opsins (e.g. NpHR3.0, Chrimson, ...). 

The system includes: 


Optional items:


Accessories:


 

Fluorescence recording
Optogenetic stimulation
Field of View650 µm x 650 µm
Working distance adjustment range

0 - 300 µm

Frame rateup to 45 fps
Pc connectionUSB 3.0
Computeur requirementsDesktop Computer recommended, Intel Core-i7, 8 GB RAM, Graphic card with 2 GB memory and OpenGL v4.6 compatible
Excitation458 / 35 nm
Emission525 / 40 nm
Wavelength612 / 69 nm
Activation intensity0 to 55 mW / mm2

The Red Fluorescence + Blue Opsins efocus microscopy system Gen 3 is optimized for calcium imaging of Red Fluorescence Proteins and activation of Blue Opsins (ChR2). 

The system includes: 


Optional items:


Accessories:


 

Fluorescence recording
Optogenetic stimulation
Field of View650 µm x 650 µm
Working distance adjustment range

0 - 300 µm

Frame rateup to 45 fps
Pc connectionUSB 3.0
Computeur requirementsDesktop Computer recommended, Intel Core-i7, 8 GB RAM, Graphic card with 2 GB memory and OpenGL v4.6 compatible
Excitation540/ 15 nm
Emission609 / 57 nm
Wavelength465 / 20 nm

The RCaMP efocus microscopy system Gen 3 is optimized for calcium imaging Red Fluorescence Proteins. 

The system includes: 


Optional items:


Accessories:


 

Fluorescence recording
Field of View 650 µm x 650 µm
Working distance adjustment range

0 - 300 µm
Frame rate up to 45 fps
Pc connection USB 3.0
Computeur requirements Desktop Computer recommended, Intel Core-i7, 8 GB RAM, Graphic card with 2 GB memory and OpenGL v4.6 compatible
Excitation 540 / 15 nm
Emission 609 / 57 nm

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