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The 3rd generation efocus Fluorescence Microscope enables calcium imaging with cellular resolution over a larger brain area of freely-moving behaving animals. Its field of view has increased to 650 x 650 µm, and the electronic depth adjustment is 300 µm. A more sensitive sensor of the 3rd generation microscope requires less fluorescence excitation light, reducing photobleaching and phototoxicity. The microscope body attaches to the imaging cannula with a simple barrel rotation. No tools are required.

Notes

  • The electrical and optical fiber tethers of an efocus Fluorescence Microscope Body Gen 3 are the same length.
  • Every microscope body comes with a protective cap.
  • The efocus Fluorescence Microscope Body Gen 3 is only compatible with the Twist-on efocus Imaging Cannulas.
Field of View650 µm x 650 µm
Working distance adjustment range 0 - 300 µm
Frame rate> 47 fps
Dimensions10 mm x 15 mm x 22 mm
Mass3 g
Computer specifications- Intel Core-i7
- 16GB RAM
- Gigabit Ethernet, Jumbo frame compatible
- Dedicated graphic card
Fluorescence recording
Green fluorescence bodies- Excitation 458 / 35 nm
- Emission 525 / 40 nm
Red fluorescence bodies- Excitation 540 / 15 nm
- Emission 609 / 57 nm
Optogenetic stimulation*
Green fluorescence bodies- spectral bandwith 612 / 69 nm
- activation intensity 0 to 55 mW/mm2
Red fluorescence bodies- spectral bandwith 465 / 20 nm
*Fluorescence + optogenetic microscopes only
Drawing

efocus Fluorescence Microscope Body

User Manual FR EN

efocus Fluorescence Microscope System Gen 3

Application Note FR EN

GCaMP configuration filter set

RCaMP2 configuration filter set

GCaMP-NpHR3 configuration filter set

GCaMP-ChR2 configuration filter set



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