efocus Fluorescence Microscope Body Gen 3

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  • The 3rd generation efocus Fluorescence Microscope enables calcium imaging with cellular resolution over a larger brain area of freely-moving behaving animals. Its field of view has increased to 650 x 650 µm, and the electronic depth adjustment is 300 µm. A more sensitive sensor of the 3rd generation microscope requires less fluorescence excitation light, reducing photobleaching and phototoxicity. The microscope body attaches to the imaging cannula with a simple barrel rotation. No tools are required.

    Notes

    • The electrical and optical fiber tethers of an efocus Fluorescence Microscope Body Gen 3 are the same length.
    • Every microscope body comes with a protective cap.
    • The efocus Fluorescence Microscope Body Gen 3 is only compatible with the Twist-on efocus Imaging Cannulas.
  •    Field of View  650 µm x 650 µm
       Working distance adjustment range 0 - 300 µm
       Frame rate > 47 fps
       Dimensions  10 mm x 15 mm x 22 mm
       Mass  3 g
       Computer specifications  - Intel Core-i7
     - 16GB RAM
     - Gigabit Ethernet, Jumbo frame compatible
     - Dedicated graphic card
    Fluorescence recording
       Green fluorescence bodies - Excitation 458 / 35 nm
    - Emission 525 / 40 nm
       Red fluorescence bodies - Excitation 540 / 15 nm
    - Emission 609 / 57 nm
    Optogenetic stimulation*
       Green fluorescence bodies - spectral bandwith 612 / 69 nm
    - activation intensity 0 to 55 mW/mm2
       Red fluorescence bodies - spectral bandwith 465 / 20 nm
    *Fluorescence + optogenetic microscopes only
  • efocus Fluorescence Microscope Body
      Drawing
    GCaMP configuration filter set
      Application Note
    RCaMP2 configuration filter set
      Application Note
    GCaMP/NpHR configuration filter set
      Application Note
    RCaMP/ChR2 configuration filter set
      Application Note