4 ports Fluorescence Mini Cube - FRET

4 ports Fluorescence Mini Cube - FRET
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Ordering Code:
  • FRET YFP-CFP recordings start with the donor fluorophore excitation with a 420-445 nm wavelength band. The excited donor fluorophores lose part of that energy to fluorescence in the 460-500 nm band. The rest goes to acceptor fluorophores in a distance-dependent, radiationless manner. The acceptor then emits the fluorescence in the 528-556 nm window.

    E is for excitation at the cube's schematic, F1 and F2 are for two spectrally different fluorescences, and S is for the fixed sample port. The numbers in the brackets are for the corresponding wavelength bands.

  • Mini Cube
    Wavelength range 350 to 1100 nm
    Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
    Spectral bandwidth CFP / YFP FRET :
    - Excitation 420-445 nm
    - Emission 460-500 nm & 528-556 nm
    Optical filter attenuation > OD 5 outside band
    Dimensions 57 mm x 63 mm x 26 mm
    Built-in Detectors
    Detector Sensitivity - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
    Amplification 3 levels : 1x, 10x, 100x
    Transimpedance gain - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    - 2.0 E11 V / A (100x)
    Conversion gain
    @ 550 nm
    - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    - 76.0 V / nW (100x)
    Saturation level
    @ 550 nm
    - 7.2 nW (1x)
    - 0.72 nW (10x)
    - 72 pW (100x)
    NEP < 12 fW/√Hz
    Bandwidth - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
    Electrical interface BNC
    Output Voltage 0.0 to 5.5 V
    Output impedance 50 Ω
    Sensor type Silicon photodiode
    Sensor Area 1 x 1 mm
    Input voltage 12 V DC (power supply included)
    Built-in LEDs
    Max Current 500 mA
    Emitter size 400 µm diameter - NA0.22
    Max Output Power
    (fibre 400 µm)
    LED 420-445 nm
    = 1.5 to 120 µW (adjustable)

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      FC connector on all ports
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    iFMC / ilFMC_GEN2 USER MANUAL
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