Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube

Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube

Mini Cube of the Rotary Bundle-imaging fiber photometry (RBFMC-G2) system contain all the LEDs, CMOS camera, dichroic mirrors and filters required for photometry excitation and detection. A core feature of these mini cubes is they can be fixed at the bottom of the RBFMC-G2 providing signal detection during the rotation and completely abolishing motion artifacts. In addition, different configurations of mini cubes can be swapped on the same RBFMC-G2 base. Note that additional mini cubes are sold separately.

Mini Cube Configurations:

Green and red photometry
Green photometry and red optogenetics*Custom cube can be made upon request.

Ordering Code =


    …generating

Green and red photometry
Green photometry and red optogenetics*Custom cube can be made upon request.

	GCaMP + RFP	GCaMP + Red Opto
Wavelength range	350 to 1100 nm
Field of view	Ø1.30 mm
Objective NA	0.50
Maximum number of sites * could be limited by patchcord manufacturing	- 7x core 400 µm NA0.57 - 20x core 200 µm NA0.57 - 90x core 100 µm NA0.37
Excitation Uniformity	10% over FOV
Optical fiber compatibility	- Core diameter 100, 200, or 400 µm - NA 0.37 to 0.57
Spectral bandwidth	- Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 555-570 nm - Emission #1 : 500-550 nm - Emission #2 : 580-680n	- Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 580-650 nm - Emission #1 : 500-540 nm
Optical filter attenuation	> OD 5 outside band
Optical fiber connector	SMA
Dimensions	102 mm x 102 mm x 346mm
SENSOR
Type	CMOS Image sensor
Resolution	540x 540 px
Pixel Size	6.9µm x6.9 µm
Frame rate	up to60Hz (1 excitation) up to 30 Hz (2 excitations)
Computer interface	USB 3.0
Build-in LEDs
Max Current	500 mA (LED 400-410 nm or 410-415 nm or 421-433 nm or 433-445 nm, LED 460-490 nm)
	1000 mA (LED 555-570 nm)	1000 mA (LED Opto 600-640 nm)
Max Output Power (400 µm fiber, NA 0.57)	LED 400-410 nm or 410-415 nm = 60µW (at 200 mA, CW)
	LED 460-490 nm = 230 µW (at 200 mA, CW)
	LED 555-570 nm = 150 µW (at 200 mA, CW)	LED Opto 600-640 nm = 7 mW (at 1000 mA, CW)
Additional Optogenetic site
Connector	M8
Max Current	1000 mA
Electrophysiology
Connector	PZN12

Drawing
Rotary Bundle-imaging Fluorescence Mini Cubes - Cube

Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube