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The purely optogenetic systems for behaving animals consist of a controlled light source, a light delivery fiber-optic patch cords, and a Fiber-optic Cannula. An additional connectorised Fiber-optic Rotary Joint can be add if the system is used on freely moving animals, to transfer the light signals and prevents the twisting of a fiber-optic tether.
The type of the optogenetic system depends on the kinds of opsins, the number of brain sites and the type of illumination.
The number of brain site depend of the experiment. In the case you choose More than 2 sites on the animal, prefer the 1 site option and a change in the fiber optic patchcord for a Bundle fiber optic patchcord.
The second option to choose is the Light source or the Light sources. By default, we present systems with a blue light source typically use for ChR2 opsin, but other wavelength could be used for other opsin. Do not hesitate to mention the wavelength needed in the comment of your request.
The other choice is LED or LD (laser diode) to make the illumination. Both have advantages and cons but the most important of them are:
- LD has a higher power by surface ratio but less output light angle (NA)
- LED are more uniform and do not present speckle profiles like the LD
| LED (NA 0.63) |
LD (NA 0.22) |
 |
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| Uniform |
Speckle |
 |
 |
For optogenetic applications, the LISER light source is the most versatile one that delivers sufficient power levels of blue or yellow incoherent light for all multi-mode optical fibers.
If the light source need to be compatible with photometry system, it is preferable to chose LED light source which are also used in fiber photometry systems.
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The Blue LED Optogenetic System allows excitation of the cells at 470 nm with a LED Light Source.
The Blue LED Optogenetic System includes :
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LED Fiber Light Source for LED light source (be changed to LED Driver and Connectorized LED for more flexibility)
- Patch Cord 240µm NA0.63 plastic/plastic (with different possibilities for the animal connector, can be changed to another fiber optic material or fiber type on request)
Rotary Joint option :
2 sites option:
For this option, we highly recommended using a rotary joint for freely moving animals.
Change compared to unilateral illumination:
Optional items:
Accessories:
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The Blue LD Optogenetic System allows excitation of the cells at 450 or 473 nm depending on the LD chosen.
The Blue LD Optogenetic System includes:
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Laser Diode Fiber Light Source for LD light source which is more powerful (can be changed to LD Driver and Connectorized Laser Diode for more flexibility)
- Patch Cord 200µm NA0.22 silica/silica (with different possibilities for the animal connector, can be changed to another fiber optic material or fiber type on request)
Rotary joint option:
2 sites option:
For this option, we highly recommended using a rotary joint for freely moving animals.
Change compared to unilateral illumination with rotary joint:
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1x2i Fiber-optic Rotary Joint with Holder
- 2x Patch Cord 200µm NA0.22 silica/silica (with different possibilities for the animal connector, can be changed to another fiber optic material or fiber type on request)
Optional items:
Accessories:
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The LISER + Blue LED Optogenetic System allows excitation of the cells at 470 nm with the LED and between 525 nm and 612 nm depending of the filter for the LISER Light source.
The LISER + Blue LED Optogenetic System includes:
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LISER Light Source with LED 470nm Light Source
- Patch Cord 240µm NA0.63 plastic/plastic (with different possibilities for the animal connector, can be changed to another fiber optic material or fiber type on request)
Rotary joint option:
2 sites option:
For this option, we highly recommended using a rotary joint for freely moving animals.
Change compared to unilateral illumination:
- 1x2 Fiber-optic Rotary Joint
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1 x Patch Cord 240µm NA0.63 plastic/plastic(with different possibilities for the animal connector, can be changed to another fiber optic material or fiber type on request)
- 1 x Patch Cord 240µm NA0.63 plastic/plastic FC-FC to connect the rotary joint and the light source
Optional items:
Accessories:
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The LISER + Blue LD Optogenetic System allows excitation of the cells at 450 or 473 nm depending on the LD chosen and between 525 nm and 612 nm depending of the filter for the LISER Light source.
The LISER + Blue LD Optogenetic System includes:
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LISER Light Source with LD Light Source (450 nm or 470 nm)
- Patch Cord 200µm NA0.22 silica/silica (with different possibilities for the animal connector, can be changed to another fiber optic material or fiber type on request)
Rotary joint option:
2 sites option:
For this option, we highly recommended using a rotary joint for freely moving animals.
Change compared to unilateral illumination:
- 1x2 Fiber-optic Rotary Joint
-
1 x Patch Cord 200µm NA0.22 silica/silica (with different possibilities for the animal connector, can be changed to another fiber optic material or fiber type on request)
- 1 x Patch Cord 200µm NA0.22 silica/silica FC-FC to connect the rotary joint and the light source
Optional items:
Accessories:
