4 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP
4 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP
4 ports Bundle-imaging Fluorescence Mini Cube (BFMC4) features 2 excitation ports with LED light sources, one detector port with CMOS sensor, and a sample port with SMA fiber optic receptacle for fiber bundle.
It combines GCaMP's isosbestic excitation with 400-410, 410-420, 421-433, or 433-445 nm light and fluorescence excitation with 460-490nm light. The CMOS 500-550nm detection window records both isosbestic and functional fluorescence signals. The separation of these signals is possible when their excitation signals are interleaved. The field of view of its microscope objective is 2.5mm, which limits applicable bundle diameter.
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4 ports Bundle-imaging Fluorescence Mini Cube (BFMC4) features 2 excitation ports with LED light sources, one detector port with CMOS sensor, and a sample port with SMA fiber optic receptacle for fiber bundle.
It combines GCaMP's isosbestic excitation with 400-410, 410-420, 421-433, or 433-445 nm light and fluorescence excitation with 460-490nm light. The CMOS 500-550nm detection window records both isosbestic and functional fluorescence signals. The separation of these signals is possible when their excitation signals are interleaved. The field of view of its microscope objective is 2.5mm, which limits applicable bundle diameter.
| Wavelength range | 350 to 1100 nm |
| Field of view | 2.5 mm |
| Objective NA | 0.40 |
| Maximum number of sites * could be limited by patchcord manufacturing | - 20x core 400 um NA0.37 - 60x core 200 um NA0.37 - 100x core 100 um NA0.37 |
| Excitation Uniformity | 10% over FOV |
| Optical fiber compatibility | - Core diameter 100, 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth | - Excitation #1 : 400-410, 410-420, 421-433, or 433-445 nm - Excitation #2 : 460-490 nm - Emission : 500-550 nm |
| Optical filter attenuation | > OD 5 outside band |
| Optical fiber connector | SMA |
| Dimensions | |
| SENSOR | |
| Type | CMOS Image sensor |
| Quantum Efficiency | 82% at 520 nm |
| Resolution | 1024 x 1024 px |
| Pixel Size | 5.86µmx5.86 µm |
| Analog gain | 0 to 72 dB |
| Frame rate | up to 60 Hz |
| Computer interface | USB 3.0 |
| Power consumption | max 200 mA (supplied by USB) |
| LEDs | |
| Output Power (fibre 200 µm) | LED 400-410 nm = 4 - 400 µW |
| LED 460-490 nm = 5 - 1000 µW | |
| Output Power (fibre 400 µm) | LED 400-410 nm = 15- 1500 µW |
| LED 460-490 nm = 20 - 4000 µW | |
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