4 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP

4 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP
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  • 4 ports Bundle-imaging Fluorescence Mini Cube (BFMC4) features 2 excitation ports with LED light sources, one detector port with CMOS sensor, and a sample port with SMA fiber optic receptacle for fiber bundle.

    It combines GCaMP's isosbestic excitation with 400-410nm or 410-420nm light and fluorescence excitation with 460-490nm light. The CMOS 500-550nm detection window records both isosbestic and functional fluorescence signals. The separation of these signals is possible when their excitation signals are interleaved. The field of view of its microscope objective is 2.5mm, which limits applicable bundle diameter.

  •    Wavelength range   350 to 1100 nm
       Field of view   2.5 mm
       Objective NA   0.40
    Maximum number of sites
    * could be limited by patchcord manufacturing
    - 7x core 400 um NA0.57
    - 20x core 400 um NA0.37
    - 60x core 200 um NA0.37
    - 100x core 100 um NA0.37
       Excitation Uniformity   10% over FOV
       Optical fiber compatibility - Core diameter 100, 200 or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth - Excitation #1 : 400-410 or 410-420 nm
    - Excitation #2 : 460-490 nm
    - Emission : 500-550 nm
       Optical filter attenuation > OD 5 outside band
       Optical fiber connector   SMA
       Dimensions  
    SENSOR
       Type   CMOS Image sensor
       Quantum Efficiency
      82% at 520 nm
       Resolution
      1024 x 1024 px
       Pixel Size
      5.86 µm x 5.86 µm
       Analog gain
      0 to 72 dB
       Frame rate
      up to 140 Hz
       Computer interface   USB 3.0 
       Power consumption   max 200 mA (supplied by USB)
    LEDs
       Output Power
       (fibre 200  µm)
      LED 400-410 nm = 4 - 400  µ
      LED 460-490 nm = 5 - 1000  µW
       Output Power
       (fibre 400  µm)
      LED 400-410 nm = 15- 1500  µW
      LED 460-490 nm = 20 - 4000  µW
  • 4 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP
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