Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube
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Mini Cube of the Rotary Bundle-imaging fiber photometry (RBFMC-G2) system contain all the LEDs, CMOS camera, dichroic mirrors and filters required for photometry excitation and detection. A core feature of these mini cubes is they can be fixed at the bottom of the RBFMC-G2 providing signal detection during the rotation and completely abolishing motion artifacts. In addition, different configurations of mini cubes can be swapped on the same RBFMC-G2 base. Note that additional mini cubes are sold separately.
Mini Cube Configurations:
1. Green and red photometry
2. Green photometry and red optogenetics
*Custom cube can be made upon request.
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GCaMP + RFP GCaMP + Red Opto Wavelength range 350 to 1100 nm Field of view Ø1.30 mm Objective NA 0.50 Maximum number of sites
* could be limited by patchcord manufacturing- 7x core 400 µm NA0.57
- 20x core 200 µm NA0.57
- 90x core 100 µm NA0.37Excitation Uniformity 10% over FOV Optical fiber compatibility - Core diameter 100, 200, or 400 µm
- NA 0.37 to 0.57Spectral bandwidth - Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
- Excitation #2 : 460-490 nm
- Excitation #3 : 555-570 nm
- Emission #1 : 500-550 nm
- Emission #2 : 580-680n
- Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
- Excitation #2 : 460-490 nm
- Excitation #3 : 580-650 nm
- Emission #1 : 500-540 nmOptical filter attenuation > OD 5 outside band Optical fiber connector SMA Dimensions 102 mm x 102 mm x 346mm SENSOR Type CMOS Image sensor
Resolution
540 x 540 px
Pixel Size
6.9 µm x 6.9 µm
Frame rate
up to 60 Hz (1 excitation)
up to 30 Hz (2 excitations)
Computer interface USB 3.0
Build-in LEDs Max Current 500 mA (LED 400-410 nm or 410-415 nm or 421-433 nm or 433-445 nm, LED 460-490 nm)
1000 mA (LED 555-570 nm)
1000 mA (LED Opto 600-640 nm) Max Output Power
(400 µm fiber, NA 0.57)LED 400-410 nm or 410-415 nm = 60 µW (at 200 mA, CW)
LED 460-490 nm = 230 µW (at 200 mA, CW)
LED 555-570 nm = 150 µW (at 200 mA, CW)
LED Opto 600-640 nm = 7 mW (at 1000 mA, CW) Additional Optogenetic site Connector M8
Max Current 1000 mA Electrophysiology Connector PZN12 -
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