Rotary Fiber Photometry System
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Fluorescence recording of freely moving tethered lab animals is challenging for neuroscience researchers due to rotary joints' transmission losses and motion-related signal variation. A Fluorescence Mini Cube with integrated LEDs mounted on an adapted 24-channel Assisted Electrical Rotary Joint rotor increases the system's overall transmission and removes motion-related artifacts.
A central hole or lumen on the rotary joint can pass an optical fiber for optogenetics stimulation or/and a tube for liquid delivery.
On request, the fluorescence Mini Cube can be adapted to other light sources, such as laser diodes and to different fluorophore combinations.
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The Rotary Fluorescence Mini Cube for GCaMP has 4 ports, two integrated LEDs, and one integrated Fluorescence Detector. It records calcium-independent GCaMP fluorescence, excited by 405 nm light (isosbestic point), and its calcium-dependent fluorescence, excited by 470 nm light, with a single photodetector. The calcium-dependent GCaMP's fluorescence emission is separated from calcium-independent emission by lock-in amplifier detection or by interleaved excitations.
The Rotary Fiber Photometry System for GCaMP includes:
- Fiber Photometry Console, the core of the system
- 2-channels LED Driver to control LED power
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Assisted Rotary-Joint with Holder
- Rotary Fluorescence Mini Cube - GCaMP with built-in LEDs and DETECTOR
- Doric Neuroscience Studio software
Optional items:
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with parts pre-assembled
- Optical Power meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
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Low-autofluorescence optical patch cords to bring light to the animal
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for ferrule to ferrule interconnect
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The Rotary Fluorescence Mini Cube with 6 ports-FRET GCaMP Isosbestic & Functional Excitations, & Red Fluorophore Fluorescence contains three integrated LED and two integrated Fluorescence Detectors. This cube can be used to detect the fluorescence from two calcium indicators and the GCaMP isosbestic point. The separation of functional and isosbestic fluorescnece signals of GCaMP is possible if the light source are modulated using lock-in demodulation or sequential detection.
The Rotary Fiber Photometry System for GCaMP and red fluorophore includes:
- Fiber Photometry Console, the core of the system
- 2-channels LED Driver to control LED power
- Assisted Rotary-Joint with Holder
- Rotary Fluorescence Mini Cube - GCaMP and red fluorophore with built-in LEDs and DETECTORs
- Doric Neuroscience Studio software
Optional items:
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with parts pre-assembled
- Optical Power meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
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Low-autofluorescence optical patch cords to bring light to the animal
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for ferrule to ferrule interconnect
- Red Laser light source or Ce:YAG
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The Rotary Fluorescence Mini Cube with 4 ports FRET CFP/YFP contains an excitation LED at 420 nm with two integrated Fluorescence Detectors for ratiometric measurement of the 520 and 550 nm emissions.
The Rotary Fiber Photometry System for FRET CFP/YFP includes:
- Fiber Photometry Console, the core of the system
- 2-channels LED Driver to control LED power
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Assisted Rotary-Joint with Holder
- Rotary Fluorescence Mini Cube - FRET with built-in LEDs and DETECTOR
- Doric Neuroscience Studio software
Optional items:
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with parts pre-assembled
- Optical Power meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
- Low-autofluorescence optical patch cords to bring light to the animal
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for ferrule to ferrule interconnect
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Behavior Camera: Synchronization and Recording
- Application Note
Rotary Fluorescence Mini Cube System
- Getting Started
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