Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube

Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube

Mini Cube of the Rotary Bundle-imaging fiber photometry (RBFMC-G2) system contain all the LEDs, CMOS camera, dichroic mirrors and filters required for photometry excitation and detection. A core feature of these mini cubes is they can be fixed at the bottom of the RBFMC-G2 providing signal detection during the rotation and completely abolishing motion artifacts. In addition, different configurations of mini cubes can be swapped on the same RBFMC-G2 base. Note that additional mini cubes are sold separately.

Mini Cube Configurations:

Green and red photometry
Green photometry and red optogenetics*Custom cube can be made upon request.

Ordering Code =


        …generating

Green and red photometry
Green photometry and red optogenetics*Custom cube can be made upon request.

	GCaMP + RFP	GCaMP + Red Opto
Wavelength range	350 to 1100 nm
Field of view	Ø1.30 mm
Objective NA	0.50
Maximum number of sites * could be limited by patchcord manufacturing	- 7x core 400 µm NA0.57 - 20x core 200 µm NA0.57 - 90x core 100 µm NA0.37
Excitation Uniformity	10% over FOV
Optical fiber compatibility	- Core diameter 100, 200, or 400 µm - NA 0.37 to 0.57
Spectral bandwidth	- Excitation #1 : 405-410 nm or 410-415 nm or 421-433 nm or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 555-570 nm - Emission #1 : 500-550 nm - Emission #2 : 580-680n	- Excitation #1 : 405-410 nm or 410-415 nm or 421-433 nm or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 580-650 nm - Emission #1 : 500-540 nm
Optical filter attenuation	> OD 5 outside band
Optical fiber connector	SMA
Dimensions	102 mm x 102 mm x 346mm
SENSOR
Type	CMOS Image sensor
Resolution	540 x 540 px
Pixel Size	6.9 µm x 6.9 µm
Frame rate	up to 60Hz (1 excitation) up to 30 Hz (2 excitations)
Computer interface	USB 3.0
Build-in LEDs
Max Current	500 mA (LED 400-410 nm or 410-415 nm or 421-433 nm or 433-445 nm, LED 460-490 nm)
	1000 mA (LED 555-570 nm)	1000 mA (LED Opto 600-640 nm)
Max Output Power (400 µm fiber, NA 0.57)	LED 400-410 nm or 410-415 nm = 60 µW (at 200 mA, CW)
	LED 460-490 nm = 230 µW (at 200 mA, CW)
	LED 555-570 nm = 150 µW (at 200 mA, CW)	LED Opto 600-640 nm = 7 mW (at 1000 mA, CW)
Additional Optogenetic site
Connector	M8
Max Current	1000 mA
Electrophysiology
Connector	PZN12

Drawing
Rotary Bundle-imaging Fluorescence Mini Cubes - Cube

Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube