Voluscan - G3

The VoluScan™ is a high-speed, mutli-plane epifluorescence microscope that recordings neural activity from thousands of neurons across a three-dimensional structure. The VoluScan™ achieves this by imaging a very large FOV (3mm x 5mm) with cellular resolution over multi-plane, all while preserving a the sampling rate required to resolve neural activity (up to 20Hz).

This solution is ideal for live-cell calcium imaging of organoids and assembloids, and provides a powerful tool to study neural circuit development and neuronal network dynamics, with cell-type specificity.

Each VoluScan™ includes a multi-plane disc that determines the number of imaging planes (4 or 8), the z-stack steps, and the total imaging depth. By default, the z-stack increments are available in 50 µm, 100 µm or 150 µm steps (others on custom request). These discs are interchangeable to fit the needs of different experiments and can be purchased separately.

The gen.3 of this system includes new targeted stimulation feature, where a specific region of the sample is selectively illuminated for optogenetics and/or uncagging experiements. This feature is particularly useful for assembloid connectivity assays, allowing the simultaneous stimulation of a organoid subregions while imaging the response of neighbouring synaptically-connected regions.

The microscope is also well suited for investigating neurodevelopmental and neurodegenerative disorders using patient-derived organoids and assembloids. The motorized 3D stage automates imaging across multiple wells, dishes, or sample regions, enabling unattended, overnight data acquisition. This high-throughput workflow accelerates drug discovery and preclinical screenings.

The microscope system comes free with Doric Neuroscience Studio, the data acquisition software. Optional addition danse™ data analysis software license, includes find cell operations (suite 2p), heatmap, optogenetic response, cross-correlation and more.

NOTE:

• An upright version of the microscope for whole cortex, zebra fish, calcium imaging etc. is available upon request.