4 ports RFMC - GCaMP
4 ports RFMC - GCaMP
The 4 ports Rotary Fluorescence Mini Cube (RFMC) supports Isosbestic & Functional GCaMP excitations and recordings of respective fluorescences. It is an add-on to the rotor of a dedicated version of our 24-channel Assisted Electrical Rotary Joint. Replacing an optical rotary joint with an electrical one increases collection efficiency and reduces motion-related signal variation.
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The 4 ports Rotary Fluorescence Mini Cube (RFMC) supports Isosbestic & Functional GCaMP excitations and recordings of respective fluorescences. It is an add-on to the rotor of a dedicated version of our 24-channel Assisted Electrical Rotary Joint. Replacing an optical rotary joint with an electrical one increases collection efficiency and reduces motion-related signal variation.
- This RFMC has two built-in LED light sources set to low excitation power.
- The integrated detector has an amplified photodiode circuit similar to that of the Doric Fluorescence Detector.
- An LED Driver and a Fluorescence Detector Amplifier connect to Rotary FMC via the Assisted Electrical Rotary Joint's stationary part.
- The RFMC's are easily attached to or removed from a rotary joint. They can be ordered separately or added to an existing system.
| Rotary Mini Cube | |
| Wavelength range | 350 to 1100 nm |
| Optical fiber compatibility | - Core diameter 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth | - Excitation : 460-490 nm Emission : 500-550 nm |
| Optical filter attenuation | > OD 5 outside band |
| Dimensions | 57 mm x 63 mm x 26 mm |
| Built-in Detectors | |
| Detector Sensitivity | - 0.38 A/W @ 550 nm - 0.60 A/W @ 960 nm (peak) |
| Amplification | 3 levels : 1x, 10x, 100x |
| Transimpedance gain | - 2.0 E09 V / A (1x) - 2.0 E10 V / A (10x) - 2.0 E11 V / A (100x) |
| Conversion gain @ 550 nm | - 0.76 V / nW (1x) - 7.60 V / nW (10x) - 76.0 V / nW (100x) |
| Saturation level @ 550 nm | - 7.2 nW (1x) - 0.72 nW (10x) - 72 pW (100x) |
| NEP | < 12 fW/√Hz |
| Bandwidth | - DC : 0 - 1000 Hz - AC : 30 - 1000 Hz |
| Electrical interface | BNC |
| Output Voltage | 0.0 to 5.5 V |
| Output impedance | 50 Ω |
| Sensor type | Silicon photodiode |
| Sensor Area | 1 x 1 mm |
| Input voltage | 12 V DC (power supply included) |
| Built-in LEDs | |
| Max Current | 500 mA |
| Emitter size | 400 µm diameter - NA0.22 |
| Output Power (fibre 200 µm) | LED 400-410 nm = 60 µW |
| LED 460-490 nm = 90 µW | |
| Output Power (fibre 400 µm) | LED 400-410 nm = 220 µW |
| LED 460-490 nm = 375 µW | |