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Doric's Bundle-imaging Fiber Photometry System (BFMC) is fully integrated, offering a hassle-free, plug-and-play solution. To record green and red photometry signals, the BFMC uses built-in LEDs to illuminate the entire sample port, and a CMOS sensor to simultaneously image the fluorescent signal from every fiber within the bundle. The fluorescent light collected from each fiber within the bundle creates circular spots on a CMOS sensor. The electrical read-out from pixels within each fiber image correlates with the calcium activity of the corresponding brain site.
The BFMC system is equipped with 8 Digital Input/Output (DIO) ports and a HD25 adapter for synchronization and closed-loop experiments with external devices such as behavior cameras, operant conditioning chambers, video tracking software, and optogenetic light sources.
To combine bundle photometry with optogenetics see Doric BFTO systems.
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The 1-colors Bundle-imaging Fiber Photometry System records activity-independent green indicator (i.e. GCaMP) fluorescence, excited by 405, 415, 425, or 440 nm light (isosbestic point), and its activity-dependent fluorescence, excited by 470 nm light, from several fiber-connected brain sites with a CMOS image sensor. The sites labelled with green indicators can be on the same animal or distributed over many animals. The respective excitations are interleaved or sequentially applied to separate activity-dependent and activity-independent fluorescence emissions.
The system includes:
- Bundle-imaging Fiber Photometry System
- All required electrical cables and patch cord
Optional items:
Accessories:
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The 2-colors Bundle-imaging Fiber Photometry System records activity-independent and activity-dependent fluorescence from a green indicator (i.e. GCaMP) and red fluorophore (reference or indicator). The signals are collected from several fiber-connected brain sites with two CMOS image sensors for the green and red channels. The excitation wavelength for the isosbestic reference can be selected between 405, 415, 425, or 440 nm, while the excitation wavelengths are 470 nm for the green channel and 560 nm for the red channel. The sites labelled with fluorophores can be on one animal or distributed over many animals. The respective fluorophore excitations are interleaved or sequentially applied to separate different fluorescence emissions.
The system includes:
- Bundle-imaging Fiber Photometry System
- All required electrical cables and patch cord
Optional items:
Accessories:
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| Mini Cube: |
| Wavelength range |
350 to 1100 nm |
| Field of view |
Ø2.5 mm |
| Objective NA |
0.40 |
Maximum number of sites
* could be limited by patchcord manufacturing |
- 20x core 400 µm NA0.37
- 60x core 200 µm NA0.37
- 100x core 100 µm NA0.37 |
| Excitation Uniformity |
10% over FOV |
| Optical fiber compatibility |
Core diameter 100, 200 or 400 µm NA 0.37 |
| Optical filter attenuation |
OD 5 outside band |
| Optical fiber connector |
SMA |
| 1-color (GCaMP) |
| Spectral bandwidth |
- Excitation #1 : 400-410, 410-420, 421-433, or 433-445 nm
- Excitation #2 : 460-490 nm
- Emission : 500-550 nm
|
| 2-colors (GCaMP + RCaMP) |
| Spectral bandwidth |
- Excitation #1 :400-410, 410-420, 421-433, or 433-445 nm
- Excitation #2 : 460-490 nm
- Excitation #3 : 555-570 nm
- Emission #1 : 500-540 nm
- Emission #2 : 580-680 nm
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| Sensor |
| Type |
CMOS Image sensor |
| Quantum Efficiency |
82% at 520 nm |
| Resolution |
1024 x 1024 px |
| Pixel Size |
5.86 µm x 5.86 µm |
| Frame rate |
up to 60 Hz |
| Build-in LEDs |
Max Current
|
500 mA |
Max Output Power
(fibre 400 µm, NA0.37)
|
LED 400-410 nm = 700 µW (at 200 mA, CW)
LED 460-490 nm = 700 µW (at 200 mA, CW)
LED 555-570 nm = 130 µW (at 200 mA, CW)
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DI/O
|
| Number of port |
32
|
| Maximum sampling rate |
10 kSps |
| Maximum output frequency |
- 8 BNC I/O,
- 1 DB25 with 8 Input/Output, 8 Input, and 8 Output
(DB25 adapter to wires included) |
Computer Requirement
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Operating System
|
Microsoft Windows 10; 64-bit |
| Memory |
8 GB RAM minimum
16 GB RAM recommended |
| Processor speed |
3 GHz and 8 cores |
| Hard Drive |
500 MB of free hard disk space>
* SSD recommended
|
| Connection to computer |
USB-3 (cable included) |
| Physical properties |
Size
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310x310x66mm |
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| Drawing |
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Bundle-imaging Fiber Photometry System Gen 3
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User ManualFrEn
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Bundle-imaging Fiber Photometry System Gen3
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