Rotary Fluorescence Mini Cube - Single Fluorophore (3 ports)

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  • Fluorescence recording of freely moving subjects is a challenge for neuroscience researchers due to low signal and external sources of noise. Here we present a modified version of our Doric Integrated Fluorescence Mini Cube that can be mounted on the rotor of a specialized version of our 24-channel Assisted Electrical Rotary Joint. This configuration increases collection efficiency by removing one optical connection while reducing motion related signal variation.

      • LED light sources are built directly into the fluorescence mini cube, and are optimized to deliver low excitation power.
      • The integrated detector is an amplified photodiode circuit with comparable specifications to our Doric Fluorescence Detector
      • To connect these devices to a LED Driver and a Fluorescence Detector Amplifier, a 24-channel Assisted Electrical Rotary Joint for Fluorescence Mini Cube is required. Each rotary joint can hold up to two rotary joints.
      • The Rotary Fluorescence Mini Cubes are modular, allowing themto be removed from a rotary joint and replaced. They can be ordered individually or added onto an existing system for added flexibility.

    The Rotary Fluorescence Mini Cube with 3 ports-For GFP-Like Fluorophore contains one integrated LED and 1 Integrated Fluorescence Detector.

  • Rotary Mini Cube
       Wavelength range   350 to 1100 nm
       Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth - Excitation : 460-490 nm
    Emission : 500-550 nm
       Optical filter attenuation > OD 5 outside band
       Dimensions   57 mm x 63 mm x 26 mm
    Built-in Detectors
       Detector Sensitivity - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
       Amplification   3 levels : 1x, 10x, 100x
       Transimpedance gain - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    - 2.0 E11 V / A (100x)
       Conversion gain
       @ 550 nm
    - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    - 76.0 V / nW (100x)
       Saturation level
       @ 550 nm
    - 7.2 nW (1x)
    - 0.72 nW (10x)
    - 72 pW (100x)
       NEP < 12 fW/√Hz
       Bandwidth - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
       Electrical interface   BNC
       Output Voltage   0.0 to 5.5 V
       Output impedance   50 Ω
       Sensor type   Silicon photodiode
       Sensor Area   1 x 1 mm
       Input voltage   12 V DC (power supply included)
    Built-in LEDs
       Max Current   200 mA
       Emitter size   400 µm diameter - NA0.22
       Output Power
       (fibre 200  µm)
      LED 400-410 nm = 60 µ
      LED 460-490 nm = 90 µW
       Output Power
       (fibre 400  µm)
      LED 400-410 nm = 220 µW
      LED 460-490 nm = 375 µW
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