4 ports Fluorescence Mini Cube - Single fluorophore with Optogenetics stimulation
4 ports Fluorescence Mini Cube - Single fluorophore with Optogenetics stimulation
This cube is for measurements involving excitation, optogenetic activation/silencing, fluorescence detection, and sample ports. Such a cube can be used for green fluorescence measurements combined with the activation of red opsins in the 580-650 nm band.
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This cube is for measurements involving excitation, optogenetic activation/silencing, fluorescence detection, and sample ports. Such a cube can be used for green fluorescence measurements combined with the activation of red opsins in the 580-650 nm band.
E stand for excitation at the cube's schematic, F for fluorescence, O for opsin activation/silencing, and S is for the sample port. The numbers in the brackets of the ordering code are for the corresponding wavelength bands.
Compared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, the versions where LEDs are integrated as well reduce the number of optical connections and the system’s overall footprint.
| Mini Cube | |
|---|---|
| Wavelength range | 350 to 1100 nm |
| Optical fiber compatibility | - Core diameter 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth | - Excitation : 460-490 nm - Emission : 500-540 nm - Optogenetics : 580-680 nm |
| Optical filter attenuation | > OD 5 outside band |
| Dimensions | 57 mm x 63 mm x 26 mm |
| Built-in Detectors | |
| Detector Sensitivity | - 0.38 A/W @ 550 nm - 0.60 A/W @ 960 nm (peak) |
| Amplification | 3 levels : 1x, 10x, 100x |
| Transimpedance gain | - 2.0 E09 V / A (1x) - 2.0 E10 V / A (10x) - 2.0 E11 V / A (100x) |
| Conversion gain @ 550 nm | - 0.76 V / nW (1x) - 7.60 V / nW (10x) - 76.0 V / nW (100x) |
| Saturation level @ 550 nm | - 7.2 nW (1x) - 0.72 nW (10x) - 72 pW (100x) |
| NEP | < 12 fW/√Hz |
| Bandwidth | - DC : 0 - 1000 Hz - AC : 30 - 1000 Hz |
| Electrical interface | BNC |
| Output Voltage | 0.0 to 5.5 V |
| Output impedance | 50 Ω |
| Sensor type | Silicon photodiode |
| Sensor Area | 1 x 1 mm |
| Input voltage | 12 V DC (power supply included) |
| Built-in LEDs (ilFMC Gen.2) | |
| Max Current | 500 mA |
| Emitter size | 400 µm diameter - NA0.22 |
| Max Output Power (fibre 400 µm) | LED 460-490 nm = 1.5 to 120 µW (adjustable) |