8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Blue and Red Optogenetics (OBSOLETE)
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Performing fiber photometry on a large number of sites and animals can be inconvenient due to the large number of photoreceivers and patch cords required. By bundling the connective patch cords together and using a camera, it is possible to measure a great number of sites or animals at once. The Bundle-imaging Fluorescence Mini Cube is able to perform such measurements. This is done by imaging a fiber bundle onto a camera using a microscope objective. These otherwise function as any fluorescence mini cube.
- The excitation ports have integrated LED light sources; UV, blue and yellow excitations.
- The detector ports come with integrated CMOS sensors; green and red emission.
- Combines GCaMP's calcium dependant signal with the calcium independant (isosbestic) reference.
- Optogenetics port for blue 450 nm laser (ChR2) is connectorized with FC/PC receptacle.
- Optogenetics port for red 638 nm laser (Chrimson, Jaws) is connectorized with FC/PC receptacle.
- To accomodate large fiber bundles, an SMA fiber optic receptacle is used on the sample port.
- Each excitation source should be interleaved and synchronized with the camera acquisition
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  Wavelength range  350 to 1100 nm   Field of view  2.5 mm   Objective NA  0.40 Maximum number of sites
* could be limited by patchcord manufacturing- 20x core 400 um NA0.37
- 60x core 200 um NA0.37
- 100x core 100 um NA0.37  Excitation Uniformity  10% over FOV   Optical fiber compatibility - Core diameter 200 or 400 µm
- NA 0.37 to 0.57Â Â Spectral bandwidth GCaMP - Excitation : 400-410 nm & 460-490 nm
- Emission : 500-550 nm  Spectral bandwidth Red Fluorophore - Excitation : 555-570 nm
- Emission : 580-620 nm  Spectral bandwidth Optogenetics - Blue : 433 - 456 nm (for 450 nm laser)
- Red : 628 - 642 nm (for 638 nm laser)  Optical filter attenuation > OD 5 outside band   Optical fiber connector  SMA   Dimensions  SENSOR   Type  CMOS Image sensor
  Quantum Efficiency
 82% at 520 nm   Resolution
 1024 x 1024 px
  Pixel Size
 5.86 µm x 5.86 µm
  Analog gain
 0 to 72 dB
  Frame rate
 up to 60 Hz
  Computer interface  USB 3.0Â
  Power consumption  max 200 mA (supplied by USB)
LEDs   Max Current  500 mA   Output Power
  (fibre 200 µm) LED 400-410 nm = 4 - 400 µWÂ
 LED 460-490 nm = 5 - 1000 µW  LED 555-570 nm = 2 - 150 µW   Output Power
  (fibre 400 µm) LED 400-410 nm = 15 - 1500 µW  LED 460-490 nm = 20 - 4000 µW  LED 555-570 nm = 8 - 600 µW
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