8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Blue and Red Optogenetics

8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Blue and Red Optogenetics
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  • Performing fiber photometry on a large number of sites and animals can be inconvenient due to the large number of photoreceivers and patch cords required. By bundling the connective patch cords together and using a camera, it is possible to measure a great number of sites or animals at once. The Bundle-imaging Fluorescence Mini Cube is able to perform such measurements. This is done by imaging a fiber bundle onto a camera using a microscope objective. These otherwise function as any fluorescence mini cube.

      • The excitation ports have integrated LED light sources; UV, blue and yellow excitations.
      • The detector ports come with integrated CMOS sensors; green and red emission.
      • Combines GCaMP's calcium dependant signal with the calcium independant (isosbestic) reference.
      • Optogenetics port for blue 450 nm laser (ChR2) is connectorized with FC/PC receptacle.
      • Optogenetics port for red 638 nm laser (Chrimson, Jaws) is connectorized with FC/PC receptacle.
      • To accomodate large fiber bundles, an SMA fiber optic receptacle is used on the sample port.
      • Each excitation source should be interleaved and synchronized with the camera acquisition
     


  •    Wavelength range   350 to 1100 nm
       Field of view   2.5 mm
       Objective NA   0.40
    Maximum number of sites
    * could be limited by patchcord manufacturing
    - 7x core 400 um NA0.57
    - 20x core 400 um NA0.37
    - 60x core 200 um NA0.37
    - 100x core 100 um NA0.37
       Excitation Uniformity   10% over FOV
       Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth GCaMP
    - Excitation : 400-410 nm & 460-490 nm
    - Emission : 500-550 nm
       Spectral bandwidth Red Fluorophore - Excitation : 555-570 nm
    - Emission : 580-620 nm
       Spectral bandwidth Optogenetics - Blue : 433 - 456 nm (for 450 nm laser)
    - Red : 628 - 642 nm (for 638 nm laser)
       Optical filter attenuation > OD 5 outside band
       Optical fiber connector   SMA
       Dimensions  
    SENSOR
       Type   CMOS Image sensor
       Quantum Efficiency
      82% at 520 nm
       Resolution
      1024 x 1024 px
       Pixel Size
      5.86 µm x 5.86 µm
       Analog gain
      0 to 72 dB
       Frame rate
      up to 140 Hz
       Computer interface   USB 3.0 
       Power consumption   max 200 mA (supplied by USB)
    LEDs
       Max Current   500 mA
       Output Power
       (fibre 200  µm)
      LED 400-410 nm = 4 - 400  µ
      LED 460-490 nm = 5 - 1000  µW
      LED 555-570 nm = 2 - 150 µW
       Output Power
       (fibre 400  µm)
      LED 400-410 nm = 15 - 1500 µW
      LED 460-490 nm = 20 - 4000 µW
      LED 555-570 nm = 8 - 600 µW
  • 8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Blue and Red Optogenetics
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