6 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore
6 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore
The 6 ports Bundle-imaging Fluorescence Mini Cube (BFMC6) features 3 excitation ports with LED light sources, 2 detector ports with CMOS sensors, and sample port with SMA fiber optic receptacle for fiber bundle.
…generating
The 6 ports Bundle-imaging Fluorescence Mini Cube (BFMC6) features 3 excitation ports with LED light sources, 2 detector ports with CMOS sensors, and sample port with SMA fiber optic receptacle for fiber bundle.
It combines GCaMP's isosbestic excitation with 400-410, 410-420, 421-433, or 433-445 nm light and fluorescence excitation with 460-490nm light. The red fluorophore is excited with 555-570nm light. The CMOS detection window for GCaMP fluorescence is from 500 to 540nm and 580 to 680nm for Red fluorophore. The separation of detected isosbestic and functional fluorescence signals is possible if their excitation signals are interleaved. The field of view of its microscope objective is 2.5 mm, which limits applicable bundle diameter.
| Wavelength range | 350 to 1100 nm |
| Field of view | 2.5 mm |
| Objective NA | 0.40 |
| Maximum number of sites * could be limited by patchcord manufacturing | - 20x core 400 um NA0.37 - 60x core 200 um NA0.37 - 100x core 100 um NA0.37 |
| Excitation Uniformity | 10% over FOV |
| Optical fiber compatibility | - Core diameter 100, 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth | - Excitation #1 : 400-410, 410-420, 421-433, or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 555-570 nm - Emission #1 : 500-540 nm - Emission #2 : 580-680 nm |
| Optical filter attenuation | > OD 5 outside band |
| Optical fiber connector | SMA |
| Dimensions | |
| SENSOR | |
| Type | CMOS Image sensor |
| Quantum Efficiency | 82% at 520 nm |
| Resolution | 1024 x 1024 px |
| Pixel Size | 5.86µm x5.86 µm |
| Analog gain | 0 to 72 dB |
| Frame rate | up to 60 Hz |
| Computer interface | USB 3.0 |
| Power consumption | max 200 mA (supplied by USB) |
| LEDs | |
| Output Power (fibre 200µm) | LED 400-410 nm = 4 - 400 µW |
| LED 460-490 nm = 5 - 1000 µW | |
| LED 555-570 nm = 2 - 150 µW | |
| Output Power (fibre 400µm) | LED 400-410 nm = 15 - 1500 µW |
| LED 460-490 nm = 20 - 4000 µW | |
| LED 555-570 nm = 8 - 600 µW | |