6 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore

6 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore
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  • 6 ports Bundle-imaging Fluorescence Mini Cube (BFMC6) features 3 excitation ports with LED light sources, 2 detector ports with CMOS sensors, and sample port with SMA fiber optic receptacle for fiber bundle.

    It combines GCaMP's isosbestic excitation with 400-410nm or 410-420nm light and fluorescence excitation with 460-490nm light. The red fluorophore is excited with 555-570nm light. The CMOS detection window for GCaMP fluorescence is from 500 to 540nm and 580 to 680nm for Red fluorophore. The separation of detected isosbestic and functional fluorescence signals is possible if their excitation signals are interleaved. The field of view of its microscope objective is 2.5 mm, which limits applicable bundle diameter.

  •    Wavelength range   350 to 1100 nm
       Field of view   2.5 mm
       Objective NA   0.40
      Maximum number of sites
      * could be limited by patchcord manufacturing
    - 7x core 400 um NA0.57
    - 20x core 400 um NA0.37
    - 60x core 200 um NA0.37
    - 100x core 100 um NA0.37
       Excitation Uniformity   10% over FOV
       Optical fiber compatibility - Core diameter 100, 200 or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth - Excitation #1 : 400-410 or 410-420 nm
    - Excitation #2 : 460-490 nm
    - Excitation #3 : 555-570 nm
    - Emission #1 : 500-540 nm
    - Emission #2 : 580-680 nm
       Optical filter attenuation > OD 5 outside band
       Optical fiber connector   SMA
       Dimensions  
    SENSOR
       Type   CMOS Image sensor
       Quantum Efficiency
      82% at 520 nm
       Resolution
      1024 x 1024 px
       Pixel Size
      5.86 µm x 5.86 µm
       Analog gain
      0 to 72 dB
       Frame rate
      up to 140 Hz
       Computer interface   USB 3.0 
       Power consumption   max 200 mA (supplied by USB)
    LEDs
       Output Power
       (fibre 200 µm)
      LED 400-410 nm = 4 - 400  µ
      LED 460-490 nm = 5 - 1000  µW
      LED 555-570 nm = 2 - 150  µW
       Output Power
       (fibre 400 µm)
      LED 400-410 nm = 15 - 1500  µW
      LED 460-490 nm = 20 - 4000  µW
      LED 555-570 nm = 8 - 600  µW
  • 6 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore
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