7 ports Fluorescence Mini Cube - GCaMP
7 ports Fluorescence Mini Cube - GCaMP
This cube combines the detection of two calcium indicators (GCaMP and red fluorophores) with the activation of red opsins. It integrates dichroic mirrors to combine fluorophores excitations, isolate the fluorescence emissions, and let opsin stimulation light through. At the same time, narrow bandpass filters limit the excitation and detection spectral bands:
…generating
This cube combines the detection of two calcium indicators (GCaMP and red fluorophores) with the activation of red opsins. It integrates dichroic mirrors to combine fluorophores excitations, isolate the fluorescence emissions, and let opsin stimulation light through. At the same time, narrow bandpass filters limit the excitation and detection spectral bands:
- 400-410 nm, 410-420 nm, 421-433 nm, or 433-445 nm for GCaMP isosbestic point excitation
- 460-490 nm for GCaMP excitation
- 500-540 nm for GCaMP fluorescence detection
- 555-570 nm for red fluorophore excitation
- 580-600 nm for red fluorescence detection
- 628-642 nm for red opsins activation
Compared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, in the latest version of Mini Cubes, LEDs and LED drivers are integrated as well to reduce the number of connections and the system’s overall footprint. In his version, it is also possible to replace the excitation filter (to adjust the isosbestic excitation from 405 nm to 410 nm for instance) and decrease the excitation power by adding attenuating filters (for low power photometry).
| Mini Cube | |
|---|---|
| Wavelength range | 350 to 1100 nm |
| Optical fiber compatibility | - Core diameter 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth | GCaMP : - Isosbestic Excitation 400-410 nm or 410-420 nm or 421-433 nm, or 433-445 nm - Excitation 460-490 nm - Emission 500-540 nm Red Fluorophore : - Excitation 555-570 nm - Emission 580-600 nm Optogenetic : - Excitation 628-642 nm |
| Optical filter attenuation | > OD 5 outside band |
| Dimensions | - FMC: 114 x 59 x 26 mm - iFMC: 127 x 92 x 50 mm - ilFMC: 152 x 109 x 52 mm |
| Built-in Detectors | iFMC | ilFMC |
|---|---|---|
| Detector Sensitivity | - 0.38 A/W @ 550 nm - 0.60 A/W @ 960 nm (peak) | - 0.38 A/W @ 550 nm - 0.60 A/W @ 960 nm (peak) |
| Amplification | 3 levels : 1x, 10x, 100x | 2levels : 1x, 10x |
| Transimpedance gain | - 2.0 E09 V / A (1x) - 2.0 E10 V / A (10x) - 2.0 E11 V / A (100x) | - 2.0 E09 V / A (1x) - 2.0 E10 V / A (10x) |
| Conversion gain @ 550 nm | - 0.76 V / nW (1x) - 7.60 V / nW (10x) - 76.0 V / nW (100x) | - 0.76 V / nW (1x) - 7.60 V / nW (10x) |
| Saturation level @ 550 nm | - 7.2 nW (1x) - 0.72 nW (10x) - 72 pW (100x) | - 7.2 nW (1x) - 0.72 nW (10x) |
| NEP | < 12 fW/√Hz | < 12 fW/√Hz |
| Bandwidth | - DC : 0 - 1000 Hz - AC : 30 - 1000 Hz | - DC : 0 - 1000 Hz - AC : 30 - 1000 Hz |
| Electrical interface | BNC | BNC |
| Output Voltage | 0.0 to 5.5 V | 0.0 to 5.5 V |
| Output impedance | 50 Ω | 50 Ω |
| Sensor type | Silicon photodiode | Silicon photodiode |
| Sensor Area | 1 x 1 mm | 1 x 1 mm |
| Input voltage | 12 V DC (power supply included) | 12 V DC (power supply included) |
| Built-in LEDs (ilFMC Gen.3) | |
| Max Current | 500 mA |
| Emitter size | 400 µm diameter - NA0.22 |
| Max Output Power (fibre 400 µm) | LED 400-410 nm = 60µW (at 200 mA, CW)* |
| LED 460-490 nm = 100 µW (at 200 mA, CW)* | |
| LED 540-570 nm = 100 µW (at 200 mA, CW) | |
* with the ND filter T=10% included.