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Optogenetics experiments often require delivering virus-born opsins to targeted cells or neurons. Typical Optical fiber Single-shot Fluid Injection Cannulas with code name OsFC has an optical fiber and a side tubing pre-loaded with a fluid and virus-born opsins. The liquid is injected after the cannula implantation surgery. Upon the first injection, the fluid passage could be clogged, and for this reason, a second injection is not recommended.
 

This cannula connects to the liquid delivery system with a flexible tube that attaches to the metal tube on the cannula.

There are several options for Fiber Length and Tubing Protrusion. Tubing Protrusion is defined relative to the optical fiber distal end, i.e., for equal length, tubing protrusion is 0 mm.

Notes:
 

  • Only Flat, Conical (C45, C60), and DFL tips are offered with this product.
  • A 2-meter length of polyethylene tubing for connecting a fluid delivery system to the cannula is sold separately.
  • Stereotaxic Cannula Holders and Receptacle adapters are available for securing the Single-shot Fluid Injection Cannula during the implantation.
Optical ConnectorCM3
Optical Transmission> 80 %
Fluid Connector
Stainless tube 485 µm OD
(compatible with 25ga tubing)
Fluid Tubing ID100 µm inner diameter
Dead Volume110 nL + 8 nL / mm fiber length
Base Dimensionsee drawing
Mass0.5 g
Drawing

Single-shot Fluid Injection Cannulas

User ManualFREN

Optofluid Cannulas Implantation and Fluid Injection



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