FluoPulse Cube
FluoPulse Cube
FluoPulse™ Cube is one of the core components of the FluoPulse (Fluorescence lifetime fiber photoemtry (FLiP)) system. The cube contains optics, excitation lasers, and photodetectors for high temporal precision lifetime measurements. Two cubes (1-color) can be driven simultaneously by a single FluoPulse™ console.
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FluoPulse™ Cube is one of the core components of the FluoPulse (Fluorescence lifetime fiber photoemtry (FLiP)) system. The cube contains optics, excitation lasers, and photodetectors for high temporal precision lifetime measurements. In addition, the cube comes with swappable bandpass and neutral density filters. Two 1-color configuration cubes can be driven by a single FluoPulse™ console.
Users can customize the FluoPulse™ Cube according to experimental needs (see Biosensor Tab):
- Up to three excitation lasers (405, 450, 488nm)
- Up to two detection windows
- cyan: 475-525 nm
- green: 500-540 nm
- red: 580-600 nm*
- custom**
- Optional Optogenetic port (628-642mm) for a 638nm laser
For simplicity and optimal measurements, we recommend using the FluoPulse™ Calibration Sample when calibrating the system.
* In the configuration with red fluorophore, please note that Doric does not provide the 561 nm laser source. Contact us for more details on the laser requirements.
**contact us for custom excitation window and excitation lasers, e.g. yellow, far-red & infrared
Here is a list of some of the most common fluorescence lifetime biosensors. In particular, we've highlighted the biosensors that have already been successfully used by clients with the FluoPulse™ system. Note that we've also included some bioRxiv papers showcasing latest innovations.
This paper, also has a more exhaustive summary: https://www.mdpi.com/2079-6374/13/10/939 but it's from 2023.
*An ideal biosensor should have an intensity change under two-fold, for optimal FluoPulse™ detector range (prevent saturation) and for accurate lifetime measures. Generally, FRET-FLIM biosensors meet this criterion, while biosensors that were generated from intensity biosensors do not. When using biosensors with large intensity changes, care must be taken to avoid saturation (adjust titre, detector gain and excitation power).
FluoPulse™ Cube
| Built-in detectors | |
|---|---|
| Wavelength detection range F1 | 500 to 540 nm |
| Wavelength detection range F2 | 580 to 600 nm |
| Built-in laser diodes | |
| Excitation wavelength options | 405, 450, 488 nm |
| Maximum Average Power | - 40 µW @ 405 nm - 30 µW @ 450 nm - 20 µW @ 488 nm |
| Pulse Duration | 450 ps |
| Pulse Repetition Rate | 400 kHz |
| Fiber connection | |
| Optical fiber core diameter | 200 or 400 µm |
| NA | 0.37 |
| Optical fiber port connector | Sample Port:FC/APC Optogenetic port (optionnal): FC/PC |
| Physical properties | |
| Dimensions Cube (width x depth x height) without knobs, connectors | 165 x 76 x 51 mm |
| Mass Cube | 950 g |
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