Bundle-imaging Fiber Photometry with Targeted Optogenetics System
Bundle-imaging Fiber Photometry with Targeted Optogenetics System
The Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics (BFTO) enables multi-site measurements through High-density Fiber-optic Patch Cords (HDP). These patch cords are available in either square or hexagonal patterns of 7, 9 or 19 fibers connected to the sample port, allowing combined optogenetics and photometry recording.
The Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics (BFTO) enables multi-site measurements through High-density Fiber-optic Patch Cords (HDP). These patch cords are available in either square or hexagonal patterns of 7, 9 or 19 fibers connected to the sample port, allowing combined optogenetics and photometry recording.
Build-in LEDs combine Isosbestic & Functional GCaMP excitations with Red Fluorophore excitation. Isosbestic and functional fluorescent signals are recorded with the same image sensor by interleaving the excitations. Both the LED driver and the CMOS detector are integrated within the system. The optogenetics stimulation pattern can vary in type, number and emitted wavelengths. For this purpose, a second similar high-density fiber optic patch cord is connected to the optogenetic port and fans out into separate connectors. Then, up to 19 independent optogenetic stimulation inputs can be connected depending on the needs of the experiment (one input per fiber connected to the sample port).
The BFTO system with targeted optogenetics supports the following fluorophore and opsin combinations:
| Fluorophores | |||
| Green Fluorophore Exc 460-490nm / Em 500-540nm | Red Fluorophore Exc 555-570nm / Em 580-600nm | ||
| Opsins | ChR2 | N/A | Sequential |
| NpHR/Chrimson | Simultaneous or Sequential | Simultaneous or Sequential | |
*560/590 on request
| Wavelength range | 350 to 1100 nm |
| Field of view | Ø3 mm |
| Objective NA | 0.40 |
| Maximum number of sites* | - 7x core 400 µm NA0.37 - 9x core 400 µm NA0.37 - 19x core 200 µm NA0.37 |
| LEDs Excitation Uniformity | 80% over FOV |
| Optogenetics Excitation Uniformity | 65% over FOV |
| Optogenetics Crosstalk between fibers | >31 dB |
| Optical fiber compatibility | - Core diameter 200, or 400 µm - NA 0.37 |
| Spectral bandwidth | - Excitation #1 : 405-410 or 410-415 nm - Excitation #2 : 460-490 nm - Excitation #3 : 555-570 nm - Emission #1 : 500-540 nm - Emission #2 : 580-600 nm |
| Optical filter attenuation | > OD 5 outside band |
| Optical fiber connector | HD7, HD9 or HD19 |
| Dimensions | 220mm x 220mm x 59mm |
| SENSOR | |
| Type | CMOS image sensor |
| Resolution | 1024x1024 px |
| Pixel size | 5.86 µm x 5.86 µm |
| Frame rate | up to 60 Hz |
| Computer interface | USB 3.0 |
| Drawing | ||
Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics | ||
| User Manual | FR | EN |
Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics | ||
The Bundle-imaging Fiber Photometry with Targeted Optogenetics System contains all items necessary to perform fiber photometry measurements on a large number of sites with green or red fluorophores on the same detector. It also features an optical fiber input port to connect to red or blue opsin activation or inhibition light source.
The system includes:
- Behavior & Bundle Photometry Console
Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics
- 4-channel LD Fiber Light Source with appropriate wavelength
- Branching box (HD19 or HD7 or HD9 depending on the choice made in the selection)
- All required electrical cables and patch cord
Optional items:
- Fiber Photometry Cannula Holder to record during surgery
- Optical Power meter to monitor and calibrate excitation power
- Behavior Camera or CamLoop to record animal behavior
- Workstation
- danse™ analyzing software
Accessories:
- High-density Fiber-optic cannula and patch cord for multiple fibers in the same animal with high placing precision chronic implant
High-density Fiber-optic Patch Cords - Fan-out and canula to record many animals simultaneously.