8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP
8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP
Performing fiber photometry on a large number of sites and animals can be inconvenient due to the large number of photoreceivers and patch cords required. By bundling the connective patch cords together and using a camera, it is possible to measure a great number of sites or animals at once. The Bundle-imaging Fluorescence Mini Cube is able to perform such measurements. This is done by imaging a fiber bundle onto a camera using a microscope objective. These otherwise function as any fluorescence mini cube.
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Performing fiber photometry on a large number of sites and animals can be inconvenient due to the large number of photoreceivers and patch cords required. By bundling the connective patch cords together and using a camera, it is possible to measure a great number of sites or animals at once. The Bundle-imaging Fluorescence Mini Cube is able to perform such measurements. This is done by imaging a fiber bundle onto a camera using a microscope objective. These otherwise function as any fluorescence mini cube.
- The excitation ports have integrated LED light sources; UV, blue and yellow excitations.
- The detector ports come with integrated CMOS sensors; green and red emission.
- Combines GCaMP's calcium dependant signal with the calcium independant (isosbestic) reference.
- Optogenetics port for blue 450 nm laser (ChR2) is connectorized with FC/PC receptacle.
- Optogenetics port for red 638 nm laser (Chrimson, Jaws) is connectorized with FC/PC receptacle.
- To accomodate large fiber bundles, an SMA fiber optic receptacle is used on the sample port.
- Each excitation source should be interleaved and synchronized with the camera acquisition
| Wavelength range | 350 to 1100 nm |
| Field of view | 2.5 mm |
| Objective NA | 0.40 |
| Maximum number of sites * could be limited by patchcord manufacturing | - 20x core 400 um NA0.37 - 60x core 200 um NA0.37 - 100x core 100 um NA0.37 |
| Excitation Uniformity | 10% over FOV |
| Optical fiber compatibility | - Core diameter 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth GCaMP | - Excitation : 400-410 nm & 460-490 nm - Emission : 500-550 nm |
| Spectral bandwidth Red Fluorophore | - Excitation : 555-570 nm - Emission : 580-620 nm |
| Spectral bandwidth Optogenetics | - Blue : 433 - 456 nm (for 450 nm laser) - Red : 628 - 642 nm (for 638 nm laser) |
| Optical filter attenuation | > OD 5 outside band |
| Optical fiber connector | SMA |
| Dimensions | |
| SENSOR | |
| Type | CMOS Image sensor |
| Quantum Efficiency | 82% at 520 nm |
| Resolution | 1024 x 1024 px |
| Pixel Size | 5.86µm x 5.86µm |
| Analog gain | 0 to 72 dB |
| Frame rate | up to 60 Hz |
| Computer interface | USB 3.0 |
| Power consumption | max 200 mA (supplied by USB) |
| LEDs | |
| Max Current | 500 mA |
| Output Power (fibre 200 µm) | LED 400-410 nm =4 - 400µW |
| LED 460-490 nm = 5 - 1000µW | |
| LED 555-570 nm = 2 - 150 µW | |
| Output Power (fibre 400 µm) | LED 400-410 nm = 15 - 1500 µW |
| LED 460-490 nm = 20 - 4000 µW | |
| LED 555-570 nm = 8 - 600 µW | |