3 ports Fluorescence Mini Cube - Single Fluorophore

3 ports Fluorescence Mini Cube - Single Fluorophore
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  • Single excitation band fiber photometry measurements use an FMC with 3 ports: one port for the excitation light, one for the fluorescence detection, and one to connect to the sample. The cube has a dichroic mirror to let the excitation light through and separate the fluorescence emission and narrow bandpass filters to limit the excitation or fluorescence spectrum. E stands for fluorescence excitation, F for fluorescence, and S for the sample port on the cube's schematic. Currently, only Green Fluorophore (GFP-like) configurations or Red Fluorophore (RFP-like) are offered. Still, the filter set can be customized for other fluorophores on request.

    The Intensity Adjustment Ring on the 460-490 nm LED light source reduces the source power below the LED driver's range if needed for low-power fiber photometry experiments.

  • Mini Cube
       Wavelength range   350 to 1100 nm
       Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth Green Fluorophore :
    - Excitation 460-490 nm
    - Emission 500-550 nm

    Red Fluorophore :
    - Excitation 540-570 nm
    - Emission 580-680 nm
       Optical filter attenuation > OD 5 outside band
       Dimensions   57 mm x 63 mm x 26 mm
    Built-in Detectors
       Detector Sensitivity - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
       Amplification   3 levels : 1x, 10x, 100x
       Transimpedance gain - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    - 2.0 E11 V / A (100x)
       Conversion gain
       @ 550 nm
    - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    - 76.0 V / nW (100x)
       Saturation level
       @ 550 nm
    - 7.2 nW (1x)
    - 0.72 nW (10x)
    - 72 pW (100x)
       NEP < 12 fW/√Hz
       Bandwidth - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
       Electrical interface   BNC
       Output Voltage   0.0 to 5.5 V
       Output impedance   50 Ω
       Sensor type   Silicon photodiode
       Sensor Area   1 x 1 mm
       Input voltage   12 V DC (power supply included)
    Built-in LEDs
       Max Current   500 mA
       Emitter size   400 µm diameter - NA0.22
       Max Output Power
       (fibre 400  µm)
      LED 460-490 nm
    = 1.5 to 120 µW (adjustable) 
      LED 540-570 nm
    = 50 µW (fixed)
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