Bundle-imaging Fiber Photometry System

Bundle-imaging Fiber Photometry System
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  • Conventional photodiode-based fiber photometry recordings of multiple brain sites on the same animal or single sites on several animals require a recording setup for each fiber location.

    An elegant alternative to multiple site photodiode-based fiber photometry is CMOS- or sCMOS-based bundle-imaging fiber photometry. The setup resembles a fluorescence microscope with a fiber bundle end face in its focal plane. The individual fibers on the other end of the fiber bundle are fitted to the selected brain locations on a single animal or on different animals. The fluorescent light from each fiber within a bundle creates circular spots on CMOS. The electrical read-out from pixels within each of those spots or fiber images correlates with the calcium activity of the corresponding brain site.




  • The GCaMP Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on a large number of animals and sites with GFP-like fluorophores. It also measures the 405 nm (isosbestic point) excitation of GCaMP fluorescence. The GCaMP fluorescence emission can be demodulated by sequential acquisition.

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  • The GCaMP with Red Fluorophore Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on a large number of animals and sites with GFP-like fluorophores. It also measures the 405 nm (isosbestic point) excitation of GCaMP fluorescence. The GCaMP fluorescence emission can be demodulated by sequential acquisition. 

    This system includes : 
    Add optional items :
    Complete your system with :
  • Excitation wavelength 405 nm for GCaMP isosbestic excitation
    470 nm for Green fluorophore excitation
    560 nm for Red fluorophore excitation
    Field of view 2.5 x 2.5 mm
    Maximum no. of sites 7x core 400 um NA0.57
    20x core 400 um NA0.37
    60x core 200 um NA0.37
    100x core 100 um NA0.37 * exact number of site could be limited by patchcord manufacturing
    Excitation Uniformity 10% over FOV
    Sensor High sensitivity CMOS image sensor (QE 86% @ 520 nm)
    Excitation Power Range typical 5 to 500 uW per fibre
    (vary with optical fibre core and NA)
    Connectivity USB3.0 port on computer
    SMA optical fiber bundle patch cords with adjustable focus (manual)
    Digital I/O 3 x Input or programmable outputs 5 V TTL
    Sampling Rate 3 to 20 Hz interleaved 3 excitations
    up to 60 Hz for single wavelength excitation
    Software Doric Neuroscience Studio that synchronize with other Doric products as laser for optogenetics and behavior camera.
  • Behavior Camera: Synchronization & Recording
      Application Note, Version 1.0.0