Bundle-imaging Fiber Photometry System

Bundle-imaging Fiber Photometry System
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  • Conventional photodiode-based fiber photometry recordings of multiple brain sites on the same animal or single sites on several animals require a recording setup for each fiber location.

    An elegant alternative to multiple site photodiode-based fiber photometry is CMOS- or sCMOS-based bundle-imaging fiber photometry. The setup resembles a fluorescence microscope with a fiber bundle end face in its focal plane. The individual fibers on the other end of the fiber bundle are fitted to the selected brain locations on a single animal or on different animals. The fluorescent light from each fiber within a bundle creates circular spots on CMOS. The electrical read-out from pixels within each of those spots or fiber images correlates with the calcium activity of the corresponding brain site.




  • The GCaMP Bundle-imaging Fiber Photometry System records calcium-independent GCaMP fluorescence, excited by 405 nm light (isosbestic point), and its calcium-dependent fluorescence, excited by 470 nm light, from several fiber-connected brain sites with a CMOS image sensor. The sites labeled with GFP-like fluorophores can be on the same animal or distributed over many animals. The respective GCamP's excitations are interleaved or sequentially applied to separate calcium-dependent and calcium-independent fluorescence emissions.

    The system includes: 
    Optional items:
    Accessories:

    Note: Other light sources and fluorophore combinations are possible. Please enquire.

  • The GCaMP with Red Fluophore Bundle-imaging Fiber Photometry System records calcium-independent and calcium-dependent GCaMP fluorescence and Red Fluophore fluorescence from several fiber-connected brain sites with a single CMOS image sensor. Respective excitation wavelengths are 405 nm (isosbestic point), 470 nm, and 560 nm. The sites labeled with fluorophores can be on one animal or distributed over many animals. The respective fluorophore excitations are interleaved or sequentially applied to separate different fluorescence emissions. 

    The system includes: 
    Optional items:
    Accessories:

    Note: Other light sources and fluorophore combinations are possible. Please enquire.

  • GCaMP and Red Fluophore Bundle-imaging Fiber Photometry System with Optogenetics records calcium-independent and calcium-dependent GCaMP fluorescence and Red Fluophore fluorescence from several fiber-connected brain sites with a single CMOS image sensor. Respective excitation wavelengths are 405 nm (isosbestic point), 470 nm, and 560 nm. The sites labeled with fluorophores or opsins can be on one animal or distributed over many animals. The respective fluorophore excitations are interleaved or sequentially applied to separate different fluorescence emissions. 

    In addition, there are 2 ports for optogenetics laser diode light delivery; 450 nm for blue opsins (i.e., ChR2) and 638 nm for red opsins (i.e., Jaws, Chrimson). Required laser power distribution over a large field of view is obtained with an appropriate selection of BFP Optogenetic Collimators.

    The system includes: 
    Optional items:
    Accessories:

    Note: Other light sources and fluorophore combinations are possible. Please enquire.

  • Excitation wavelength 405 nm for GCaMP isosbestic excitation
    470 nm for Green fluorophore excitation
    560 nm for Red fluorophore excitation
    Field of view 2.5 x 2.5 mm
    Maximum no. of sites 7x core 400 um NA0.57
    20x core 400 um NA0.37
    60x core 200 um NA0.37
    100x core 100 um NA0.37
    * exact number of site could be limited by patchcord manufacturing
    Excitation Uniformity 10% over FOV
    Sensor High sensitivity CMOS image sensor (QE 86% @ 520 nm)
    Excitation Power Range typical 5 to 500 uW per fibre
    (vary with optical fibre core and NA)
    Connectivity USB3.0 port on computer
    SMA optical fiber bundle patch cords with adjustable focus (manual)
    Digital I/O 3 x Input or programmable outputs 5 V TTL
    Sampling Rate 3 to 20 Hz interleaved 3 excitations
    up to 60 Hz for single wavelength excitation
    Software Doric Neuroscience Studio that synchronize with other Doric products as laser for optogenetics and behavior camera.
  • Bundle-imaging Fluorescence Mini Cubes
      User Manual
    Behavior Camera: Synchronization and Recording
      Application Note