Bundle-imaging Fiber Photometry System

Bundle-imaging Fiber Photometry System
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  • Conventional photodiode-based fiber photometry recordings of multiple brain sites on the same animal or single sites on several animals require a recording setup for each fiber location.

    An elegant alternative to multiple site photodiode-based fiber photometry is CMOS- or sCMOS-based bundle-imaging fiber photometry. The setup resembles a fluorescence microscope with a fiber bundle end face in its focal plane. The individual fibers on the other end of the fiber bundle are fitted to the selected brain locations on a single animal or on different animals. The fluorescent light from each fiber within a bundle creates circular spots on CMOS. The electrical read-out from pixels within each of those spots or fiber images correlates with the calcium activity of the corresponding brain site.




  • The GCaMP Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on a large number of animals and sites with GFP-like fluorophores. It also measures the 405 nm (isosbestic point) excitation of GCaMP fluorescence. The GCaMP fluorescence emission can be demodulated by sequential acquisition.

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  • The GCaMP with Red Fluorophore Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on a large number of animals and sites with GFP-like fluorophores. It also measures the 405 nm (isosbestic point) excitation of GCaMP fluorescence and collect the red fluorescence on another camera. The fluorescence resulting of different excitation are demodulated by sequential acquisition. 

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  • The GCaMP and Red Fluorophore Bundle-imaging Fiber Photometry System with Optogenetics port at 450 and 638 nm contains all items necessary to perform fiber photometry measurements on a large number of animals and sites with GFP-like fluorophores.  It also measures the 405 nm (isosbestic point) excitation of GCaMP fluorescence and collect the red fluorescence on another sensor. The fluorescence resulting of different excitation are demodulated by sequential acquisition. 

    In addition, there is 2 lasers diodes ports for optogenetics light delivery; 450 nm for blue opsins (i.e. ChR2) and 638 nm for red opsins (i.e. Jaws, Chrimson). The laser input power is distributed into a large field of view of which is determined by the selection of the BFP Optogenetic Collimators. 

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  • Excitation wavelength 405 nm for GCaMP isosbestic excitation
    470 nm for Green fluorophore excitation
    560 nm for Red fluorophore excitation
    Field of view 2.5 x 2.5 mm
    Maximum no. of sites 7x core 400 um NA0.57
    20x core 400 um NA0.37
    60x core 200 um NA0.37
    100x core 100 um NA0.37
    * exact number of site could be limited by patchcord manufacturing
    Excitation Uniformity 10% over FOV
    Sensor High sensitivity CMOS image sensor (QE 86% @ 520 nm)
    Excitation Power Range typical 5 to 500 uW per fibre
    (vary with optical fibre core and NA)
    Connectivity USB3.0 port on computer
    SMA optical fiber bundle patch cords with adjustable focus (manual)
    Digital I/O 3 x Input or programmable outputs 5 V TTL
    Sampling Rate 3 to 20 Hz interleaved 3 excitations
    up to 60 Hz for single wavelength excitation
    Software Doric Neuroscience Studio that synchronize with other Doric products as laser for optogenetics and behavior camera.
  • Behavior Camera: Synchronization & Recording
      Application Note, Version 1.0.0