Rotary Bundle-imaging Fluorescence Photometry System (OBSOLETE)

Conventional photodiode-based fiber photometry recordings of multiple brain sites on one behaving tethered animal require an entire recording setup for each fiber location. That includes the need for fiber optic rotary joint with independent optical channels.

So far, only 2 independent channels rotary joints are available. CMOS-based Rotary Bundle-imaging fiber photometry is an elegant alternative for multiple site recording setups. The Rotary Bundle-imaging Fluorescence Mini Cube incorporates a motorized rotary joint that senses the torsion of the optical tether during animal movement and turns the cube accordingly. The assisted rotary joint counters the friction of the internal slip-ring and provides quality transfer of optical and electrical signals during experiments with freely-moving animals like mice or rats.

The whole system resembles a fluorescence microscope with a fiber bundle end face at its focal plane. The individual fibers on the other end of the fiber bundle are fitted to implanted cannulas in selected animals' brain locations. The fluorescent light from each fiber within a bundle creates a distinct circular spot on CMOS. Since CMOS detector rotates with the fiber bundle, the electrical read-out from pixels within those spots correlates with the calcium activity of the corresponding brain site and is not affected by rotation artifacts.

The GCaMP with Red Fluorophore Rotary Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on a large number of sites with GFP-like fluorophores. It also measures the isosbestic excitation of GCaMP fluorescence and collects the red fluorescence on the same sensor. The fluorescence resulting from different excitations are demodulated by sequential acquisition.
The system includes:
Optional items:
Accessories:

The GCaMP with Opto 590 nm Rotary Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on a large number of sites with GFP-like fluorophores. It also measures the isosbestic excitation of GCaMP fluorescence and collects the green fluorescence on the same sensor. The fluorescence resulting from different excitations are demodulated by sequential acquisition.
This system is also equipped to deliver the illumination for the yellow opsin at 590 nm.
The system includes:
Optional items:
Accessories:
The GCaMP with Red Fluorophore and 638 nm Red Opsin Rotary Bundle-imaging Fiber Photometry System contains all items necessary to perform fiber photometry measurements on a large number of sites with GFP-like fluorophores. It also measures the isosbestic excitation of GCaMP fluorescence and collects the red fluorescence on the same sensor. The fluorescence resulting from different excitation are demodulated by sequential acquisition.
The system is also equipped to deliver the illumination for the Red opsin at 638nm.
The system includes:
Optional items:
Accessories:

Field of view	Ø1.25 mm
Maximum number of sites * exact number of sites could be limited by patchcord manufacturing	- 7x core 400 µm NA0.57 - 19x core 200 µm NA0.57 - 90x core 100 µm NA0.37
Excitation Uniformity	10% over FOV
Sensor	High sensitivity CMOS image sensor
Connectivity	USB2.0 port on the computer SMA optical fiber bundle patch cords with adjustable focus (manual)
Digital I/O	3 x Input or programmable outputs 5 V TTL
Sampling Rate	3 to 20 Hz interleaved 3 excitations up to 60 Hz for single wavelength excitation
Software	Doric Neuroscience Studio V6 and higher

Drawing

Rotary Bundle-imaging Fluorescence Mini Cubes

User ManualFrEn

Rotary Bundle-imaging Fluorescence Mini Cubes

Brochure

Rotary Bundle Photometry System