Acute Recording System - Multi units

Acute Recording System - Multi units
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  • To better understand the functioning of the brain, it is necessary to precisely understand the activity of neurons and their network.

    Using extracellular in vivo electrophysiological recordings, it is possible to monitor neuronal activity from outside the cell. It provides a means to measure patterns of action potentials within many areas of the nervous systems.

    Since the extracellular recording are not neuron specific, we developed two kinds of systems where electrical recording is combined with two different techniques (Optogenetics and photometry).
    In both Optogenetics and photometry cells are labelled with specific fluorophores, thus providing insights at the cellular level.

  • Optogenetics and electrophysiology are combined in this system to allow recordings of synaptic events at a single-cell resolution in a head-fixed configuration. It is designed to activate optogenetic proteins such as channelrhodopsine or to inhibit light-gated ion pumps like halorhodopsin. By adjusting the illumination output power, it is possible to illuminate a single cell or a group of neurons. Extracellular electrophysiology is possible by filling the fiber probe hollow core with an electrolyte solution to get an electrode impedance in the range of 1 to 20 M, allowing a single-unit or multiunit spikes detection.

    See Recording the Illuminated Neuron animation from Biomedical Communications on Vimeo.

    This system includes specifically: