Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP
Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP
This Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations with yellow optogenetic inhibition. Isosbestic and functional fluorescence signals are recorded with the same image sensor by interleaving the excitation signals. Rotary bundle-imaging enables simultaneous recordings from all sites within Ø1.25 mm field of view, limiting the applicable bundle diameter. Consider the non-rotating Bundle-imaging Fiber Photometry Systems for a larger field of view.
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This Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations with yellow optogenetic inhibition. Isosbestic and functional fluorescence signals are recorded with the same image sensor by interleaving the excitation signals. Rotary bundle-imaging enables simultaneous recordings from all sites within Ø1.25 mm field of view, limiting the applicable bundle diameter. Consider the non-rotating Bundle-imaging Fiber Photometry Systems for a larger field of view.
| Wavelength range | 350 to 1100 nm |
| Field of view | Ø1.25 mm |
| Objective NA | 0.50 |
| Maximum number of sites * could be limited by patchcord manufacturing | - 7x core 400 µm NA0.57 - 20x core 200 µm NA0.57 - 90x core 100 µm NA0.37 |
| Excitation Uniformity | 10% over FOV |
| Optical fiber compatibility | - Core diameter 100, 200, or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth | - Excitation #1 : 405-410 or 410-415 nm - Excitation #2 : 460-490 nm - Emission #1 : 500-550 nm -Opto #1 : 580-650 nm |
| Optical filter attenuation | > OD 5 outside band |
| Optical fiber connector | SMA |
| Dimensions | 92 mm x 83 mm x 267mm |
| SENSOR | |
| Type | CMOS Image sensor |
| Resolution | 540x 540 px |
| Pixel Size | 6.9µm x6.9 µm |
| Frame rate | up to60Hz (1 excitation) up to 30 Hz (2 excitations) |
| Computer interface | USB 2.0 |