Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore
This 3 ports Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations with Red Fluorophore excitation. Isosbestic and functional fluorescence signals are recorded on a single integrated image sensor by interleaving the excitation signals. By bundling the connective patch cords together and using a camera, it is possible to measure a great number of sites at once. The field of view is Ø1.25 mm, which limits applicable bundle diameter. For a larger field of view, consider the non-rotating Bundle-imaging Fiber Photometry Systems.
The RBFMCs are easily attached to or removed from a dedicated assisted rotary joint. The assisted rotary joint for BFMC can be ordered separately or added to an existing system.
Other rotary joints and bundle-imaging fluorescent mini cubes are not compatible with rotary bundle fluorescent mini cubes devices.
Wavelength range 350 to 1100 nm Field of view Ø1.25 mm Objective NA 0.50 Maximum number of sites
* could be limited by patchcord manufacturing
- 7x core 400 µm NA0.57
- 20x core 200 µm NA0.57
- 90x core 100 µm NA0.37
Excitation Uniformity 10% over FOV Optical fiber compatibility - Core diameter 100, 200, or 400 µm
- NA 0.37 to 0.57
Spectral bandwidth - Excitation #1 : 405-410 or 410-415 nm
- Excitation #2 : 460-490 nm
- Excitation #3 : 555-570 nm
- Emission #1 : 500-540 nm
- Emission #2 : 580-680 nm
Optical filter attenuation > OD 5 outside band Optical fiber connector SMA Dimensions 65 mm x 39 mm x 159 mm SENSOR Type CMOS Image sensor
540 x 540 px
6.9 µm x 6.9 µm
up to 100 Hz
Computer interface USB 2.0
Rotary Bundle-imaging Fluorescence Mini Cubes
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