The FiWi Cannulas for chronic implantation into an animal's brain exist in Optoelectric, Opto, Ephys, or Test variety.
The bottom of a FiWi Optoelectric cannula consists of an optical fiber and 1 to 4 electrodes. The optical fiber glued to an LED illuminates a selected brain area, and electrodes record the area's brain activity. The LED light intensity pulse could be modulated from 0-100%. Its frequency can be varied from continuous wave (CW) up to 1 kHz. The optical fiber and the electrodes can be custom-positioned with 100 µm precision. The tungsten electrode impedance could be set between 0.2 and 1.2 MΩ.
FiWi Opto Cannula has a LED glued to a protruding optical fiber. At the same time, FiWi Ephys Cannula features only electrodes. The FiWi Test Cannula is a robust version of the FiWi Opto Cannula with a larger diameter, short optical fiber used to verify the position of the illumination pattern before implanting the fragile Opto Cannula.
At the top of the cannula is an electrical connector for attaching or disconnecting the headstage between the experiments.
RECOMMENDED USE CODE 50 µm tungsten wire with flat tip 0.1 to 0.5 M LFP W50-FLT 50 µm tungsten wire with 45 deg angled tip 0.1 to 0.5 M LFP W50-A45 75 µm tungsten wire with tapered tip 0.1 M LFP 0.1M-75 0.5 M LFP 0.5M-75 1.0 M Multi unit 1.0M-75 1.5 M Multi unit 1.5M-75 2.0 M Single and Multi unit 2.0M-75 2.5 M Single and Multi unit 2.5M-75 3.0 M Single unit 3.0M-75 5.0 M Single unit 5.0M-75 125 µm tungsten wire with tapered tip 0.1 M Deep Chronic implant, LFP 0.1M-125 0.5 M Deep Chronic implant, LFP 0.5M-125 1.0 M Deep Chronic implant, Multi unit 1.0M-125 1.5 M Deep Chronic implant, Multi unit 1.5M-125 2.0 M Deep Chronic implant, Single and Multi unit 2.0M-125 2.5 M Deep Chronic implant, Single and Multi unit 2.5M-125 3.0 M Deep Chronic implant, Single unit 3.0M-125 5.0 M Deep Chronic implant, Single unit 5.0M-125
Optical fiber - 200/250 µm - NA 0.66
- 400/430 µm - NA 0.66
LED power @ 150 mA
* fibre 200 µm - NA0.66
- 465 nm = 8.0 mW (250 mW/mm²)
- 525 nm = 3.5 mW (110 mW/mm²)
- 595 nm = 1.5 mW (50 mW/mm²)
- 635 nm = 4.0 mW (130 mW/mm²)
Electrodes (tungsten wires) - 50 μm diameter, (0.2 to 0.3 MΩ) Dimensions 10 mm x 10 mm x 7 mm Mass 0.3 g
The light propagation into brain tissue will vary with the optical fiber type and the light source parameters. It can be visualized in this Application Note (the French version is available here).
Description Drawing Code Note Flat Tip FLT Conical Tip
Rounded tip thickness: ~0.1x to 0.2x core diameter
Standard angles: 45°, 60°
Other angles on request (max 60°)
Taper Tip TAPER
Fiberless and Wireless Optogenetically Synchronized Electrophysiology System
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