Bundle imaging Fiber photometry Optogenetic Collimator

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  • Those collimators are designed to add specific optogenetics illumination capability to Bundle-Imaging Fluorescence Minicube.  The collimator model will depends on the light source as the field of view is function of the divergence of the light output from the optical fiber.  This divergence is given by the numerical aperture of the optical fiber, and how the light have been injected into it.  For example, laser are typically injected with coupler having effective NA of 0.20 and is typically used with optical fiber having NA0.22.  In another hand, the CeYAG light source is incoherent light and is highly divergent, it is used with high NA optical fibers (0.50 and +) and completely fill the optical fiber acceptance cone angle.

    Bundle
    Illumination size (mm) Recommended
    Laser FOV
    (mm)
    Recommended
    CeYAG FOV
    (mm)
    4x 200 µm 0.56 0.75 0.90
    7x 200 µm 0.60 0.75 0.90
    12x 200 µm 0.95 1.10 1.30
    19x 200 µm 1.12 1.25 1.30
    4x 400 µm 1.05 1.25 1.30
    7x 400 µm 1.20 1.40 1.30
    12x 400 µm 1.75 1.90 1.90
    19x 400 µm 2.10 2.20 3.00

    ..

  •    Wavelength range   350 to 700 nm
       Field of view   selected by table in DESCRIPTION section
       BFMC objective   10x - NA 0.40
       Optical fiber connector   FC/PC
    Table 1. General Specifications



    Type
    Source
    Effective NA
    Collimator
    Focal Length
    ( mm )
    Optogenetic port
    Field of View
    ( mm )
    'AD'
    achromatic
    doublet
    0.20 12.0
    0.75
    17.5 1.10
    20.0 1.25
    22.5 1.40
    30.0 1.90
    'AH'
    aspheric
    high NA
    0.50 3.0 0.90
    4.3 1.30
    5.5 1.60
    8.0 1.90
    12.5 3.00
    Table 2. Field of View vs collimator specifications
    *valid with BFMC objective 10x - NA0.40.
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