Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore

Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore
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  • This Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations, Red Fluorophore excitation, and optogenetic activation at 638 nm. Isosbestic and functional fluorescence signals are recorded with the same image sensor by interleaving the excitation signals. Rotary bundle-imaging enables simultaneous recordings from all sites within Ø1.25 mm field of view, limiting the applicable bundle diameter. Consider the non-rotating Bundle-imaging Fiber Photometry Systems for a larger field of view.

  •    Wavelength range   350 to 1100 nm
       Field of view   Ø1.25 mm
       Objective NA   0.50
      Maximum number of sites
      * could be limited by patchcord manufacturing
    - 7x core 400 µm NA0.57
    - 20x core 200 µm NA0.57
    - 90x core 100 µm NA0.37
       Excitation Uniformity   10% over FOV
       Optical fiber compatibility - Core diameter 100, 200, or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth - Excitation #1 : 405-410 or 410-415 nm
    - Excitation #2 : 460-490 nm
    - Excitation #3 : 555-570 nm
    - Emission #1 : 500-540 nm
    - Emission #2 : 580-680 nm
       Optical filter attenuation > OD 5 outside band
       Optical fiber connector  SMA
       Dimensions  65 mm x 39 mm x 159 mm
    SENSOR
       Type   CMOS Image sensor
       Resolution
      540 x 540 px
       Pixel Size
      6.9 µm x 6.9 µm
       Frame rate
      up to 100 Hz
       Computer interface   USB 2.0 
  • Rotary Bundle-imaging Fluorescence Mini Cubes
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