Single-shot Fluid Injection Cannulas
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Optogenetics experiments often require delivering virus-born opsins to targeted cells or neurons. Typical Optical fiber Single-shot Fluid Injection Cannulas with code name OsFC has an optical fiber and a side tubing pre-loaded with a fluid and virus-born opsins. The liquid is injected after the cannula implantation surgery. Upon the first injection, the fluid passage could be clogged, and for this reason, a second injection is not recommended.
This cannula connects to the liquid delivery system with a flexible tube that attaches to the metal tube on the cannula.
There are several options for Fiber Length and Tubing Protrusion. Tubing Protrusion is defined relative to the optical fiber distal end, i.e., for equal length, tubing protrusion is 0 mm.
Notes:
- Only Flat, Conical (C45, C60), and DFL tips are offered with this product.
- A 2-meter length of polyethylene tubing for connecting a fluid delivery system to the cannula is sold separately.
- Stereotaxic Cannula Holders and Receptacle adapters are available for securing the Single-shot Fluid Injection Cannula during the implantation.
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Optical Connector CM3 Optical Transmission > 80 % Fluid Connector Stainless tube 485 µm OD
(compatible with 25ga tubing)Fluid Tubing ID 100 µm inner diameter Dead Volume 110 nL + 8 nL / mm fiber length Base Dimension see drawing
Mass 0.5 g -
Core (μm) Outer (μm) NA Buffer Color Outer Layer Fiber-Optic Code 100 125 0.22 Yellow Polyimide
100/125-0.22 100 125 0.37 Yellow Polyimide
100/125-0.37 100 125 0.66 Clear Borosilicate
100/125-0.66 200 240 0.22 Yellow Polyimide
200/240-0.22 200 245 0.37 Yellow Polyimide
200/245-0.37 200 250 0.66 Clear Borosilicate 200/250-0.66 300 370 0.22 Yellow Polyimide
300/370-0.22 300 360 0.37 Yellow Polyimide
300/360-0.37 400 480 0.22 Yellow Polyimide 400/480-0.22 400 470 0.37 Yellow Polyimide 400/470-0.37 400 430 0.55 Clear Borosilicate
400/430-0.66 -
Drawing Single-shot Fluid Injection Cannulas
User ManualFrEn Optofluid Cannulas Implantation and Fluid Injection
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