Rotary Bundle-imaging Fluorescence Photometry System

Rotary Bundle-imaging Fluorescence Photometry System
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  • Conventional photodiode-based fiber photometry recordings of multiple brain sites on one behaving tethered animal require an entire recording setup for each fiber location. That includes the need for fiber optic rotary joint with independent optical channels.

    So far, only 2 independent channels rotary joints are available. CMOS-based Rotary Bundle-imaging fiber photometry is an elegant alternative for multiple site recording setups. The Rotary Bundle-imaging Fluorescence Mini Cube incorporates a motorized rotary joint that senses the torsion of the optical tether during animal movement and turns the cube accordingly. The assisted rotary joint counters the friction of the internal slip-ring and provides quality transfer of optical and electrical signals during experiments with freely-moving animals like mice or rats.

    The whole system resembles a fluorescence microscope with a fiber bundle end face at its focal plane. The individual fibers on the other end of the fiber bundle are fitted to implanted cannulas in selected animals' brain locations. The fluorescent light from each fiber within a bundle creates a distinct circular spot on CMOS. Since CMOS detector rotates with the fiber bundle, the electrical read-out from pixels within those spots correlates with the calcium activity of the corresponding brain site and is not affected by rotation artifacts.