Bundle-imaging Fiber Photometry System

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  • Conventional photodiode-based fiber photometry recordings of multiple brain sites on the same animal or single sites on several animals require a recording setup for each fiber location.

    An elegant alternative to multiple site photodiode-based fiber photometry is CMOS- or sCMOS-based bundle-imaging fiber photometry. The setup resembles a fluorescence microscope with a fiber bundle end face in its focal plane. The individual fibers on the other end of the fiber bundle are fitted to the selected brain locations on a single animal or on different animals. The fluorescent light from each fiber within a bundle creates circular spots on CMOS. The electrical read-out from pixels within each of those spots or fiber images correlates with the calcium activity of the corresponding brain site.

    For a Bundle Photometry option with optogenetics see: Bundle Photometry with Targeted Optogenetics (BFTO).


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