6 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluoro

6 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP with Red Fluorophore

The 6 ports Bundle-imaging Fluorescence Mini Cube (BFMC6) features 3 excitation ports with LED light sources, 2 detector ports with CMOS sensors, and sample port with SMA fiber optic receptacle for fiber bundle.

It combines GCaMP's isosbestic excitation with 400-410, 410-420, 421-433, or 433-445 nm light and ﬂuorescence excitation with 460-490nm light. The red fluorophore is excited with 555-570nm light. The CMOS detection window for GCaMP fluorescence is from 500 to 540nm and 580 to 680nm for Red fluorophore. The separation of detected isosbestic and functional fluorescence signals is possible if their excitation signals are interleaved. The field of view of its microscope objective is 2.5 mm, which limits applicable bundle diameter.

Wavelength range	350 to 1100 nm
Field of view	2.5 mm
Objective NA	0.40
Maximum number of sites * could be limited by patchcord manufacturing	- 20x core 400 um NA0.37 - 60x core 200 um NA0.37 - 100x core 100 um NA0.37
Excitation Uniformity	10% over FOV
Optical fiber compatibility	- Core diameter 100, 200 or 400 µm - NA 0.37 to 0.57
Spectral bandwidth	- Excitation #1 : 400-410, 410-420, 421-433, or 433-445 nm - Excitation #2 : 460-490 nm - Excitation #3 : 555-570 nm - Emission #1 : 500-540 nm - Emission #2 : 580-680 nm
Optical filter attenuation	> OD 5 outside band
Optical fiber connector	SMA
Dimensions
SENSOR
Type	CMOS Image sensor
Quantum Efficiency	82% at 520 nm
Resolution	1024 x 1024 px
Pixel Size	5.86 µm x 5.86 µm
Analog gain	0 to 72 dB
Frame rate	up to 60 Hz
Computer interface	USB 3.0
Power consumption	max 200 mA (supplied by USB)
LEDs
Output Power (fibre 200 µm)	LED 400-410 nm = 4 - 400 µW
	LED 460-490 nm = 5 - 1000 µW
	LED 555-570 nm = 2 - 150 µW
Output Power (fibre 400 µm)	LED 400-410 nm = 15 - 1500 µW
	LED 460-490 nm = 20 - 4000 µW
	LED 555-570 nm = 8 - 600 µW