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This Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations with yellow optogenetic inhibition. Isosbestic and functional fluorescence signals are recorded with the same image sensor by interleaving the excitation signals. Rotary bundle-imaging enables simultaneous recordings from all sites within Ø1.25 mm field of view, limiting the applicable bundle diameter. Consider the non-rotating Bundle-imaging Fiber Photometry Systems for a larger field of view.
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| Wavelength range |
350 to 1100 nm |
| Field of view |
Ø1.25 mm |
| Objective NA
|
0.50 |
Maximum number of sites
* could be limited by patchcord manufacturing |
- 7x core 400 µm NA0.57
- 20x core 200 µm NA0.57
- 90x core 100 µm NA0.37 |
| Excitation Uniformity
|
10% over FOV |
| Optical fiber compatibility |
- Core diameter 100, 200, or 400 µm
- NA 0.37 to 0.57
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| Spectral bandwidth |
- Excitation #1 : 405-410 or 410-415 nm
- Excitation #2 : 460-490 nm
- Emission #1 : 500-550 nm
- Opto #1 : 580-650 nm
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| Optical filter attenuation |
> OD 5 outside band |
| Optical fiber connector |
SMA |
| Dimensions
|
92 mm x 83 mm x 267mm |
| SENSOR |
| Type
|
CMOS Image sensor
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Resolution
|
540 x 540 px
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Pixel Size
|
6.9 µm x 6.9 µm
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Frame rate
|
up to 60 Hz (1 excitation)
up to 30 Hz (2 excitations)
|
| Computer interface
|
USB 2.0
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| Drawing |
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Rotary Bundle-imaging Fluorescence Mini Cubes
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User ManualFrEn
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Rotary Bundle-imaging Fluorescence Mini Cubes
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| Brochure |
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Rotary Bundle Photometry System
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