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Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Red Optogenetics (OBSOLETE)

Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Red Optogenetics (OBSOLETE)

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  • This Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations with Red Fluorophore excitation and optogenetic activation at 638nm. Isosbestic and functional fluorescence signals are recorded with the same image sensor by interleaving the excitation signals. Rotary bundle-imaging enables simultaneous recordings from all sites within Ø1.25 mm field of view, limiting the applicable bundle diameter. Consider the non-rotating Bundle-imaging Fiber Photometry Systems for a larger field of view.

  •    Wavelength range  350 to 1100 nm
       Field of view  Ø1.25 mm
       Objective NA   0.50
      Maximum number of sites
      * could be limited by patchcord manufacturing    		 - 7x core 400 µm NA0.57
    - 20x core 200 µm NA0.57
    - 90x core 100 µm NA0.37
       Excitation Uniformity  10% over FOV
       Optical fiber compatibility - Core diameter 100, 200, or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth

    - Excitation #1 : 405-410 or 410-415 nm
     - Excitation #2 : 460-490 nm
    - Excitation #3 : 555-570 nm
    - Emission #1 : 500-550 nm
    - Emission #2 : 580-620 nm
    - Opto #1 : 628-642 nm
       Optical filter attenuation > OD 5 outside band
       Optical fiber connector  SMA
       Dimensions 92 mm x 83 mm x 267mm
    SENSOR
       Type   CMOS Image sensor
       Resolution
    		   540 x 540 px
       Pixel Size
    		   6.9 µm x 6.9 µm
       Frame rate
    		   up to 60 Hz (1 excitation)
      up to 30 Hz (2 excitations)
      up to 12 Hz (3 excitations)
       Computer interface   USB 2.0 
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    Rotary Bundle-imaging Fluorescence Mini Cubes
    User ManualFrEn

    Rotary Bundle-imaging Fluorescence Mini Cubes
    Brochure

    Rotary Bundle Photometry System