Panier 0

6 ports RFMC - GCaMP + Red fluorophore

6 ports RFMC - GCaMP + Red fluorophore
6 ports RFMC - GCaMP + Red fluorophore


Ordering Code:

 

  • The 6 ports Rotary Fluorescence Mini Cube (RFMC) supports Isosbestic & Functional GCaMP excitations, Red Fluorophore excitation, and recordings of respective fluorescences. The separation of functional and isosbestic GCaMP fluorescence signals is possible with modulated excitations lights using lock-in demodulation or sequential detection.

    • This RFMC has 3 built-in LED light sources set to low excitation power.
    • Two integrated detectors have an amplified photodiode circuit similar to that of the Doric Fluorescence Detector.
    • A LED Drivers and Fluorescence Detector Amplifiers connect to Rotary FMC via the Assisted Electrical Rotary Joint's stationary part.
    • The RFMC's are easily attached to or removed from a rotary joint. They can be ordered separately or added to an existing system.
  • Rotary Mini Cube
       Wavelength range   350 to 1100 nm
       Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth - Excitation : 460-490 nm, 555-570 nm
    - Emission  : 500-550 nm, 580-680 nm
       Optical filter attenuation > OD 5 outside band
       Dimensions   57 mm x 63 mm x 26 mm
    Built-in Detectors
       Detector Sensitivity - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
       Amplification   3 levels : 1x, 10x, 100x
       Transimpedance gain - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    - 2.0 E11 V / A (100x)
       Conversion gain
       @ 550 nm    		 - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    - 76.0 V / nW (100x)
       Saturation level
       @ 550 nm    		 - 7.2 nW (1x)
    - 0.72 nW (10x)
    - 72 pW (100x)
       NEP < 12 fW/√Hz
       Bandwidth - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
       Electrical interface   BNC
       Output Voltage   0.0 to 5.5 V
       Output impedance   50 Ω
       Sensor type   Silicon photodiode
       Sensor Area   1 x 1 mm
       Input voltage   12 V DC (power supply included)
    Built-in LEDs
       Max Current   500 mA
       Emitter size   400 µm diameter - NA0.22
       Max LED Power (lockin)
       (fibre 200 µm)     		  400-410 nm = 60 µW 
      460-490 nm = 90 µW
      555-570 nm = 30 µW
       Max LED Power (lockin)
       (fibre 400 µm)     		  400-410 nm = 220 µW
      460-490 nm = 375 µW
      555-570 nm = 120 µW
  • Drawing

    RFMC cube
    Getting StartedFrEn

    Fiberless &Wireless Optogenetically Synchronized Electrophysiology System